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LearnMAB718-100
antibody from R&D Systems
Targeting: RET
CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET51
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- MAB718-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Ret Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Ret in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse Ret is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P07949- Isotype
- IgG
- Antibody clone number
- 132507
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function.
Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.
Fonseca-Pereira D, Arroz-Madeira S, Rodrigues-Campos M, Barbosa IA, Domingues RG, Bento T, Almeida AR, Ribeiro H, Potocnik AJ, Enomoto H, Veiga-Fernandes H
Nature 2014 Oct 2;514(7520):98-101
Nature 2014 Oct 2;514(7520):98-101
Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.
Kubota H, Wu X, Goodyear SM, Avarbock MR, Brinster RL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Aug;25(8):2604-14
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Aug;25(8):2604-14
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Ret in SH-SY5Y Human Cell Line by Flow Cytometry. SH-SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human Ret Monoclonal Antibody (Catalog # MAB718, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
