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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB4033 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4033, RRID:AB_1579833
- Product name
- YWHAB polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of YWHAB.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references 14-3-3beta is a p90 ribosomal S6 kinase (RSK) isoform 1-binding protein that negatively regulates RSK kinase activity.
Immunoexpression of 14-3-3 proteins in glial cytoplasmic inclusions of multiple system atrophy.
The p38 and MK2 kinase cascade phosphorylates tuberin, the tuberous sclerosis 2 gene product, and enhances its interaction with 14-3-3.
Cavet ME, Lehoux S, Berk BC
The Journal of biological chemistry 2003 May 16;278(20):18376-83
The Journal of biological chemistry 2003 May 16;278(20):18376-83
Immunoexpression of 14-3-3 proteins in glial cytoplasmic inclusions of multiple system atrophy.
Komori T, Ishizawa K, Arai N, Hirose T, Mizutani T, Oda M
Acta neuropathologica 2003 Jul;106(1):66-70
Acta neuropathologica 2003 Jul;106(1):66-70
The p38 and MK2 kinase cascade phosphorylates tuberin, the tuberous sclerosis 2 gene product, and enhances its interaction with 14-3-3.
Li Y, Inoki K, Vacratsis P, Guan KL
The Journal of biological chemistry 2003 Apr 18;278(16):13663-71
The Journal of biological chemistry 2003 Apr 18;278(16):13663-71
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The YWHAB polyclonal antibody (Cat # PAB4033) is used in Western blot to detect YWHAB in Jurkat cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with YWHAB polyclonal antibody (Cat # PAB4033) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
