Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- BML-SA479-0100 - Provider product page
- Provider
- Enzo Life Sciences
- Product name
- [pSer185]14-3-3, pAb
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Affinity purified phospho-specific antibody.
- Vial size
- 100 µl
Submitted references Increased 14-3-3 phosphorylation observed in Parkinson's disease reduces neuroprotective potential of 14-3-3 proteins.
Slone SR, Lavalley N, McFerrin M, Wang B, Yacoubian TA
Neurobiology of disease 2015 Jul;79:1-13
Neurobiology of disease 2015 Jul;79:1-13
No comments: Submit comment
Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis performed with BML-SA479 antibody (1:500), secondary anti-sheep antibody (1:2'000), blotted against 2µg each of: 1. Recombinant 14-3-3_ expressed in E.coli 2. Recombinant 14-3-3_ expressed in E.coli 3. Sheep brain homogenate, which contains ~50% ofÊ_ andÊ_ isoforms that are phosphorylated on Ser185.
