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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB262-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Amphiregulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Amphiregulin (AR) in Western blots. In this format, this antibody shows less than 5% cross-reactivity with recombinant human HB-EGF.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 31221
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment leading to osteoclastogenesis.
CRB3 and the FERM protein EPB41L4B regulate proliferation of mammary epithelial cells through the release of amphiregulin.
Amphiregulin carboxy-terminal domain is required for autocrine keratinocyte growth.
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Raimondo S, Saieva L, Vicario E, Pucci M, Toscani D, Manno M, Raccosta S, Giuliani N, Alessandro R
Journal of hematology & oncology 2019 Jan 8;12(1):2
Journal of hematology & oncology 2019 Jan 8;12(1):2
CRB3 and the FERM protein EPB41L4B regulate proliferation of mammary epithelial cells through the release of amphiregulin.
Walker SJ, Selfors LM, Margolis BL, Brugge JS
PloS one 2018;13(11):e0207470
PloS one 2018;13(11):e0207470
Amphiregulin carboxy-terminal domain is required for autocrine keratinocyte growth.
Stoll SW, Johnson JL, Li Y, Rittié L, Elder JT
The Journal of investigative dermatology 2010 Aug;130(8):2031-40
The Journal of investigative dermatology 2010 Aug;130(8):2031-40
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Gonzalez RM, Seurynck-Servoss SL, Crowley SA, Brown M, Omenn GS, Hayes DF, Zangar RC
Journal of proteome research 2008 Jun;7(6):2406-14
Journal of proteome research 2008 Jun;7(6):2406-14
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by Amphiregulin and Neutralization by Human Amphiregulin Antibody. Recombinant Human Amphiregulin (Catalog # 262-AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Amphiregulin (50 ng/mL) is neutralized (green line) by increasing concentrations of Human Amphiregulin Monoclonal Antibody (Catalog # MAB262). The ND50 is typically 5-15 µg/mL.
