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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- NB500-140 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-140, RRID:AB_10001082
- Product name
- Rabbit Polyclonal MARCKS Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~87k MARCKS protein phosphorylated at Ser152 and Ser156 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase
- Reactivity
- Rat, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Development of a sensitive in vitro assay to quantify the biological activity of pro-inflammatory phorbol esters in Jatropha oil.
Regulation of SR-BI-mediated selective lipid uptake in Chinese hamster ovary-derived cells by protein kinase signaling pathways.
Pelletier G, Padhi BK, Hawari J, Sunahara GI, Poon R
In vitro cellular & developmental biology. Animal 2015 Jun;51(6):644-50
In vitro cellular & developmental biology. Animal 2015 Jun;51(6):644-50
Regulation of SR-BI-mediated selective lipid uptake in Chinese hamster ovary-derived cells by protein kinase signaling pathways.
Zhang Y, Ahmed AM, McFarlane N, Capone C, Boreham DR, Truant R, Igdoura SA, Trigatti BL
Journal of lipid research 2007 Feb;48(2):405-16
Journal of lipid research 2007 Feb;48(2):405-16
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: MARCKS [p Ser152, p Ser156] Antibody [NB500-140] - Western blot of rat brain lysate showing specific immunolabeling of the ~87k MARCKS protein phosphorylated at Ser152,156 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the MARCKS Ser 152,156 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
