HPA009426

antibody from Atlas Antibodies

Targeting: DPEP1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA009426

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA009426, RRID:AB_1847842

Product name

Anti-DPEP1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

VMVNFYNNYISCTNKANLSQVADHLDHIKEVAGAR
AVGFGGDFDGVPRVPEGLEDVSKYPDLIAELLRRN
WTEAEVKGALADNLLRVFQAVEQASNLTQAPEEEP
IPLDQLGGSCRTHYGYSSGAS


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and DPEP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401402).

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human kidney and lymph node tissues using Anti-DPEP1 antibody. Corresponding DPEP1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human colon, kidney, liver and testis using Anti-DPEP1 antibody HPA009426 (A) shows similar protein distribution across tissues to independent antibody HPA012783 (B).

Antibody #2 product nr

HPA012783

Antibody provider

Atlas Antibodies

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human small intestine shows membranous positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver using Anti-DPEP1 antibody HPA009426.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon using Anti-DPEP1 antibody HPA009426.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis using Anti-DPEP1 antibody HPA009426.

Sample type

HUMAN