NBP1-79126

antibody from Novus Biologicals

Targeting: CD28

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-79126

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-79126, RRID:AB_11014852

Product name

Mouse Monoclonal CD28 Antibody

Antibody type

Monoclonal

Description

Protein A purified. The antibody CD28.2 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells. HLDA V.

Reactivity

Human, Simian

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

T Cell Receptor-Independent, CD31/IL-17A-Driven Inflammatory Axis Shapes Synovitis in Juvenile Idiopathic Arthritis.

Ferguson ID, Griffin P, Michel JJ, Yano H, Gaffen SL, Mueller RG, Dvergsten JA, Piganelli JD, Rosenkranz ME, Kietz DA, Vallejo AN
Frontiers in immunology 2018;9:1802

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PE.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PerCP-CyTM5.5

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) APC.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PerCP.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) FITC.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PE-CyTM7.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) purified.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1,6 ug/ml) GAM APC.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining pattern of human peripheral blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1,6 ug/ml) GAM APC.