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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AM26541AF-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti MDM4 pSer367
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- #15
- Vial size
- 50 µg
- Concentration
- 1.0 mg/ml
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot analysis of phosphorylated MdmX (Ser367) expression in MCF7. MCF cells were preincubated with the proteasome inhibitor MG132 (20 µM) and exposed to DNA damaging agent, adriamycin (3 µM) or etoposide (20 µM), for the indicated periods. The cell lysates were used for immunoprecipitation with anti-MdmX antibody (D-19). The immunocomplex was resolved on SDS-PAGE and immunoblotted with anti-MdmX antibody, AM26541AF-S and anti-14-3-3 à antibody.
