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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- AP52096PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52096PU-N, RRID:AB_11143123
- Product name
- anti HRAS (C-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 153-184 amino acids from the C-terminal region of human HRAS
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of HRAS Antibody (C-term) Cat.-No AP52096PU-N in mouse cerebellum tissue lysates (35ug/lane). This demonstrates the HRAS antibody detected the HRAS protein (arrow).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of HRAS (arrow) using HRAS Antibody (C-term) Cat.-No AP52096PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HRAS gene.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of HRAS Antibody (C-term) Cat.-No AP52096PU-N with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
