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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- TA504509 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504509, RRID:AB_11129415
- Product name
- FOLH1 mouse monoclonal antibody, clone OTI2E1 (formerly 2E1)
- Antibody type
- Monoclonal
- Description
- FOLH1 mouse monoclonal antibody, clone OTI2E1 (formerly 2E1)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- FOLH1
- Isotype
- IgG
- Antibody clone number
- OTI2E1
- Vial size
- 100 µl
- Concentration
- 0.84 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOLH1 (RC218310, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOLH1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-FOLH1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Standard curve for ELISA analysis with FOLH1 recombinant protein (dilution range from 5ng/ml to 5ug/ml) using FOLH1 Capture Antibody (Cat# TA504509) at 5ug/ml and HRP conjugated FOLH1 Detection mAb (Cat# TA504550) at 0.03ug/ml.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-FOLH1 mouse monoclonal antibody (TA504509) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FOLH1(RC218310).
- Validation comment
- IF
