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- Antibody Data
- Antigen structure
- References [15]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AF1027 - Provider product page
- Provider
- R&D Systems
- Product name
- Human B7-H3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human B7-H3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) B7-H2 is observed, and less than 1% cross-reactivity with rhB7-1, rhB7-2, rhB7-H1, and rhPD-L2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_079516- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references B7H3 regulates differentiation and serves as a potential biomarker and theranostic target for human glioblastoma.
B7-H3 promotes aerobic glycolysis and chemoresistance in colorectal cancer cells by regulating HK2.
B7-H3 Promotes the Migration and Invasion of Human Bladder Cancer Cells via the PI3K/Akt/STAT3 Signaling Pathway.
Spectroscopic Photoacoustic Molecular Imaging of Breast Cancer using a B7-H3-targeted ICG Contrast Agent.
High constitutive B7-H3 expression on human keratinocytes supports T cell immunity.
Preferential Induction of the T Cell Auxiliary Signaling Molecule B7-H3 on Synovial Monocytes in Rheumatoid Arthritis.
Breast Cancer Detection by B7-H3-Targeted Ultrasound Molecular Imaging.
Origination of new immunological functions in the costimulatory molecule B7-H3: the role of exon duplication in evolution of the immune system.
Anterior pituitary progenitor cells express costimulatory molecule 4Ig-B7-H3.
Anterior pituitary progenitor cells express costimulatory molecule 4Ig-B7-H3.
Interactions of T cells with fibroblast-like synoviocytes: role of the B7 family costimulatory ligand B7-H3.
Interactions of T cells with fibroblast-like synoviocytes: role of the B7 family costimulatory ligand B7-H3.
Tumor cell and tumor vasculature expression of B7-H3 predict survival in clear cell renal cell carcinoma.
12-O-tetradecanoyl phorbol 13-acetate induces the expression of B7-DC, -H1, -H2, and -H3 in K562 cells.
The immunomodulatory proteins B7-DC, B7-H2, and B7-H3 are differentially expressed across gestation in the human placenta.
Zhang J, Wang J, Marzese DM, Wang X, Yang Z, Li C, Zhang H, Zhang J, Chen CC, Kelly DF, Hua W, Hoon DSB, Mao Y
Laboratory investigation; a journal of technical methods and pathology 2019 Jul;99(8):1117-1129
Laboratory investigation; a journal of technical methods and pathology 2019 Jul;99(8):1117-1129
B7-H3 promotes aerobic glycolysis and chemoresistance in colorectal cancer cells by regulating HK2.
Shi T, Ma Y, Cao L, Zhan S, Xu Y, Fu F, Liu C, Zhang G, Wang Z, Wang R, Lu H, Lu B, Chen W, Zhang X
Cell death & disease 2019 Apr 5;10(4):308
Cell death & disease 2019 Apr 5;10(4):308
B7-H3 Promotes the Migration and Invasion of Human Bladder Cancer Cells via the PI3K/Akt/STAT3 Signaling Pathway.
Li Y, Guo G, Song J, Cai Z, Yang J, Chen Z, Wang Y, Huang Y, Gao Q
Journal of Cancer 2017;8(5):816-824
Journal of Cancer 2017;8(5):816-824
Spectroscopic Photoacoustic Molecular Imaging of Breast Cancer using a B7-H3-targeted ICG Contrast Agent.
Wilson KE, Bachawal SV, Abou-Elkacem L, Jensen K, Machtaler S, Tian L, Willmann JK
Theranostics 2017;7(6):1463-1476
Theranostics 2017;7(6):1463-1476
High constitutive B7-H3 expression on human keratinocytes supports T cell immunity.
Quandt D, Fiedler E, Müller A, Marsch WC, Seliger B
Journal of dermatological science 2017 Jul;87(1):82-85
Journal of dermatological science 2017 Jul;87(1):82-85
Preferential Induction of the T Cell Auxiliary Signaling Molecule B7-H3 on Synovial Monocytes in Rheumatoid Arthritis.
Yoon BR, Chung YH, Yoo SJ, Kawara K, Kim J, Yoo IS, Park CG, Kang SW, Lee WW
The Journal of biological chemistry 2016 Feb 19;291(8):4048-57
The Journal of biological chemistry 2016 Feb 19;291(8):4048-57
Breast Cancer Detection by B7-H3-Targeted Ultrasound Molecular Imaging.
Bachawal SV, Jensen KC, Wilson KE, Tian L, Lutz AM, Willmann JK
Cancer research 2015 Jun 15;75(12):2501-9
Cancer research 2015 Jun 15;75(12):2501-9
Origination of new immunological functions in the costimulatory molecule B7-H3: the role of exon duplication in evolution of the immune system.
Sun J, Fu F, Gu W, Yan R, Zhang G, Shen Z, Zhou Y, Wang H, Shen B, Zhang X
PloS one 2011;6(9):e24751
PloS one 2011;6(9):e24751
Anterior pituitary progenitor cells express costimulatory molecule 4Ig-B7-H3.
Nagai Y, Aso H, Ogasawara H, Tanaka S, Taketa Y, Watanabe K, Ohwada S, Rose MT, Kitazawa H, Yamaguchi T
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6073-81
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6073-81
Anterior pituitary progenitor cells express costimulatory molecule 4Ig-B7-H3.
Nagai Y, Aso H, Ogasawara H, Tanaka S, Taketa Y, Watanabe K, Ohwada S, Rose MT, Kitazawa H, Yamaguchi T
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6073-81
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6073-81
Interactions of T cells with fibroblast-like synoviocytes: role of the B7 family costimulatory ligand B7-H3.
Tran CN, Thacker SG, Louie DM, Oliver J, White PT, Endres JL, Urquhart AG, Chung KC, Fox DA
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):2989-98
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):2989-98
Interactions of T cells with fibroblast-like synoviocytes: role of the B7 family costimulatory ligand B7-H3.
Tran CN, Thacker SG, Louie DM, Oliver J, White PT, Endres JL, Urquhart AG, Chung KC, Fox DA
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):2989-98
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):2989-98
Tumor cell and tumor vasculature expression of B7-H3 predict survival in clear cell renal cell carcinoma.
Crispen PL, Sheinin Y, Roth TJ, Lohse CM, Kuntz SM, Frigola X, Thompson RH, Boorjian SA, Dong H, Leibovich BC, Blute ML, Kwon ED
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Aug 15;14(16):5150-7
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Aug 15;14(16):5150-7
12-O-tetradecanoyl phorbol 13-acetate induces the expression of B7-DC, -H1, -H2, and -H3 in K562 cells.
Jang BC, Park YK, Choi IH, Kim SP, Hwang JB, Baek WK, Suh MH, Mun KC, Suh SI
International journal of oncology 2007 Dec;31(6):1439-47
International journal of oncology 2007 Dec;31(6):1439-47
The immunomodulatory proteins B7-DC, B7-H2, and B7-H3 are differentially expressed across gestation in the human placenta.
Petroff MG, Kharatyan E, Torry DS, Holets L
The American journal of pathology 2005 Aug;167(2):465-73
The American journal of pathology 2005 Aug;167(2):465-73
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human B7-H3 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human B7-H3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1027) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for B7-H3 at approximately 90-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human B7-H3 by Simple WesternTM. Simple Western lane view shows lysates of U2OS human osteosarcoma cell line, loaded at 0.2 mg/mL. A specific band was detected for B7-H3 at approximately 120-160 kDa (as indicated) using 10 µg/mL of Goat Anti-Human B7-H3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1027) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Western Blot Shows Human B7-H3 Specificity by Using Knockout Cell Line. Western blot shows lysates of U2OS human osteosarcoma parental cell line and B7-H3 knockout U2OS cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human B7-H3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1027) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- B7-H3 in Human Melanoma. B7-H3 was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-Human B7-H3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1027) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of B7-H3 in PC-3 Human Cell Line by Flow Cytometry. PC-3 human prostate carcinoma cells were stained with Goat Anti-Human B7-H3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1027, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
