Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-49916 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-49916, RRID:AB_10012106
- Product name
- Rabbit Polyclonal Ancient ubiquitous protein 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ancient ubiquitous protein 1 Antibody [NBP1-49916] - Detection of Human AUP1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-AUP1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. AUP1 was also immunoprecipitated by rabbit anti-AUP1 antibody BL10556, which recognizes an upstream epitope. For blotting immunoprecipitated AUP1 wase used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Ancient ubiquitous protein 1 Antibody [NBP1-49916] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-AUP1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB