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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
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- Product number
- 602802 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#602802, RRID:AB_2251471
- Product name
- Purified anti-Ninein
- Antibody type
- Polyclonal
- Antigen
- Recombinant (partial), C-terminal
- Description
- Ninein (also known asGSK3β-interacting protein and ninein centrosomal protein) is a chromosome segregation ATPase with 5 reported isoforms, (molecular weights ranging from 236 to 246 kD). Ninein is localized at the centrosome and is thought to position and anchor microtubules minus-ends. Ninein is important for centrosomal function in epithelial cells. This protein is modified by phosphorylation. Ninein has also been shown to interact with CEP250/c-Nap1, CEP110, γ-tubulin, centrin, GSK3β, AIK, and PKA. The Poly6028 antibody recognizes the N-terminal region of human ninein and has been shown to be useful for Western blotting.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 200 µl
- Storage
- Upon receipt, store frozen at -20° C.
Submitted references Trichoplein controls microtubule anchoring at the centrosome by binding to Odf2 and ninein.
The retinitis pigmentosa protein RP2 interacts with polycystin 2 and regulates cilia-mediated vertebrate development.
Ninein is expressed in the cytoplasm of angiogenic tip-cells and regulates tubular morphogenesis of endothelial cells.
In-frame deletion in a novel centrosomal/ciliary protein CEP290/NPHP6 perturbs its interaction with RPGR and results in early-onset retinal degeneration in the rd16 mouse.
Ibi M, Zou P, Inoko A, Shiromizu T, Matsuyama M, Hayashi Y, Enomoto M, Mori D, Hirotsune S, Kiyono T, Tsukita S, Goto H, Inagaki M
Journal of cell science 2011 Mar 15;124(Pt 6):857-64
Journal of cell science 2011 Mar 15;124(Pt 6):857-64
The retinitis pigmentosa protein RP2 interacts with polycystin 2 and regulates cilia-mediated vertebrate development.
Hurd T, Zhou W, Jenkins P, Liu CJ, Swaroop A, Khanna H, Martens J, Hildebrandt F, Margolis B
Human molecular genetics 2010 Nov 15;19(22):4330-44
Human molecular genetics 2010 Nov 15;19(22):4330-44
Ninein is expressed in the cytoplasm of angiogenic tip-cells and regulates tubular morphogenesis of endothelial cells.
Matsumoto T, Schiller P, Dieterich LC, Bahram F, Iribe Y, Hellman U, Wikner C, Chan G, Claesson-Welsh L, Dimberg A
Arteriosclerosis, thrombosis, and vascular biology 2008 Dec;28(12):2123-30
Arteriosclerosis, thrombosis, and vascular biology 2008 Dec;28(12):2123-30
In-frame deletion in a novel centrosomal/ciliary protein CEP290/NPHP6 perturbs its interaction with RPGR and results in early-onset retinal degeneration in the rd16 mouse.
Chang B, Khanna H, Hawes N, Jimeno D, He S, Lillo C, Parapuram SK, Cheng H, Scott A, Hurd RE, Sayer JA, Otto EA, Attanasio M, O'Toole JF, Jin G, Shou C, Hildebrandt F, Williams DS, Heckenlively JR, Swaroop A
Human molecular genetics 2006 Jun 1;15(11):1847-57
Human molecular genetics 2006 Jun 1;15(11):1847-57
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- BioLegend (provider)
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- Experimental details
- MCF7 (Lane 1) and MOLT-4 (Lane 2) cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-ninein antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. In hematopoietic cells (such as MOLT-4), two additional bands with apparent molecular weights of 110 and 130 kD are observed.
- Conjugate
- Unconjugated
