MAB1039

antibody from R&D Systems

Targeting: CD244 2B4, NAIL, NKR2B4, Nmrk, SLAMF4

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1039

Provider

R&D Systems

Product name

Human 2B4/CD244/SLAMF4 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human 2B4/CD244/SLAMF4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse 2B4/CD244/SLAMF4 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

NP_057466


Isotype

IgG

Antibody clone number

146510

Vial size

500 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Shear flow-dependent integration of apical and subendothelial chemokines in T-cell transmigration: implications for locomotion and the multistep paradigm.

Schreiber TH, Shinder V, Cain DW, Alon R, Sackstein R
Blood 2007 Feb 15;109(4):1381-6

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

2B4/CD244/SLAMF4 in Human Spleen. 2B4/CD244/SLAMF4 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human 2B4/CD244/SLAMF4 Monoclonal Antibody (Catalog # MAB1039) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.