MAB9078

antibody from Novus Biologicals

Targeting: CD274 B7-H, B7-H1, B7H1, PD-L1, PDCD1LG1, PDL1

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB9078

Provider

Novus Biologicals

Product name

Rat Monoclonal PD-L1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse PD-L1/B7-H1 in direct ELISAs. Stains mouse PD-L1/B7-H1 transfectants but not irrelevant transfectants in flow cytometry.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of PD-L1/B7-H1 in HEK293 Human Cell Line Transfected with Mouse PD-L1/B7-H1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse PD-L1/B7-H1 or (B) irrelevant transfectants and eGFP was stained with and either (A) Rat Anti-Mouse PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB9078) or (B) Rat IgG_2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Hamster IgG Secondary Antibody .

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of PD-L1/B7-H1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line either treated with LPS overnight (filled histogram) or untreated (open historgram) was stained with Rat Anti-Mouse PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB9078), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.