R33263

antibody from NSJ Bioreagents

Targeting: CD274 B7-H, B7-H1, B7H1, PD-L1, PDCD1LG1, PDL1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R33263

Provider

NSJ Bioreagents

Product name

PD-L1 Antibody

Antibody type

Polyclonal

Antigen

Amino acids CKKQSDTHLEET were used as the immunogen for this PD-L1 antibody.

Description

Antigen affinity purified antibody

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

Aliquot and store the PD-L1 antibody at -20°C.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human heart lysate with PD-L1 antibody at 1ug/ml. Predicted molecular weight ~34kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human heart lysate with PD-L1 antibody at 0.1ug/ml. Predicted molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human placenta with PD-L1 antibody at 4ug/ml. HIER: steamed with pH9 Tris/EDTA buffer, HRP-staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human placenta tissue with PD-L1 antibody at 2ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.