GTX629976

antibody from GeneTex

Targeting: CANX CNX, IP90, P90

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX629976

Provider

GeneTex

Product name

Calnexin antibody [GT1563]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat

Host

Mouse

References

Submitted references

A high-throughput pipeline for validation of antibodies.

Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (15 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (GTX629976) diluted at 1:6000.

KD Reagent Type

siRNA

Downregulation

>75%

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod no

Proprietary.

Antibody Lot Number

41484

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis.A. 50 ?g mouse liver lysate/extract7.5 % SDS-PAGECalnexin antibody [GT1563] (GTX629976) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis.A. 30 ?g PC-12 whole cell lysate/extractB. 30 ?g Rat-2 whole cell lysate/extract7.5 % SDS-PAGECalnexin antibody [GT1563] (GTX629976) dilution: 1:500

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extractC. 30 ?g HeLa whole cell lysate/extractD. 30 ?g HepG2 whole cell lysate/extract7.5 % SDS-PAGECalnexin antibody [GT1563] (GTX629976) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (15 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (GTX629976) diluted at 1:6000.