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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AP21606SU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP21606SU-S, RRID:AB_10757401
- Product name
- anti BFAR / RNF47
- Antibody type
- Polyclonal
- Antigen
- A recombinant protein corresponding to amino acids 1-139 of human BAR
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 50 µl
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of BAR in cell lines and tissues. 25 µg of total protein was loaded per lane. BAR Recombinant protein (RP) was used as a positive control.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded sections of tumor and normal human tissues stained for BAR expression using AP21606SU-S at 1/2000. A. Pancreatic cancer. B. Normal pancreas. C. Esophageal cancer. D. Normal esophagus. Increased expression of BAR was detected in the tumor, compared to normal tissues. Hematoxylin-eosin counterstain.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded tissue sections of normal human brain stained for BAR expression using AP21606SU-S at 1/2000. A, A1: Cerebellum. B, B1: Medulla. A1 and B1 are higher magnifications of A and B, respectively.
