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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-27958 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NELL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32.
- Concentration
- 1.58 mg/mL
Submitted references NELL1 is a target antigen in malignancy-associated membranous nephropathy.
Caza TN, Hassen SI, Dvanajscak Z, Kuperman M, Edmondson R, Herzog C, Storey A, Arthur J, Cossey LN, Sharma SG, Kenan DJ, Larsen CP
Kidney international 2021 Apr;99(4):967-976
Kidney international 2021 Apr;99(4):967-976
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NELL1 using 30 µg of A) NT2D1 and B) IMR32 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a NELL1 polyclonal antibody (Product # PA5-27958) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NELL1 Polyclonal Antibody (Product # PA5-27958). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NELL1 Polyclonal Antibody (Product # PA5-27958) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using NELL1 (Product # PA5-27958) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
