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- Antibody Data
- Antigen structure
- References [46]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- 20582-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#20582-1-AP, RRID:AB_10695760
- Product name
- PERK/EIF2AK3 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated PERK/EIF2AK3 antibody (Cat. #20582-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, WB, ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Neuroprotective Effects of Exercise Postconditioning After Stroke via SIRT1-Mediated Suppression of Endoplasmic Reticulum (ER) Stress.
Interventional Efects of the Topical of "Sanse Powder" Essential Oils Nanoemulsion on Knee Osteoarthritis in Rats by Targeting the ERS/TXNIP/NLRP3 Signaling Axis.
Kaempferol Alleviates Steatosis and Inflammation During Early Non-Alcoholic Steatohepatitis Associated With Liver X Receptor α-Lysophosphatidylcholine Acyltransferase 3 Signaling Pathway.
Inhibition of the miR-193b-3p protects against oxidized low-density lipoprotein-induced HUVECs injury by upregulating ALDH2.
Cytotoxic BODIPY-Appended Half-Sandwich Iridium(III) Complex Forms Protein Adducts and Induces ER Stress.
Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy.
Choline attenuates heat stress-induced oxidative injury and apoptosis in bovine mammary epithelial cells by modulating PERK/Nrf-2 signaling pathway.
MCLR induces dysregulation of calcium homeostasis and endoplasmic reticulum stress resulting in apoptosis in Sertoli cells.
Microcystin-leucine arginine induced the apoptosis of GnRH neurons by activating the endoplasmic reticulum stress resulting in a decrease of serum testosterone level in mice.
Endoplasmic reticulum stress pathway mediates T-2 toxin-induced chondrocyte apoptosis.
Deficiency of β-arrestin2 alleviates apoptosis through GRP78-ATF6-CHOP signaling pathway in primary Sjögren's syndrome.
Mannan-binding lectin deficiency augments hepatic endoplasmic reticulum stress through IP3R-controlled calcium release.
Citrus alkaline extracts prevent endoplasmic reticulum stress in type II alveolar epithelial cells to ameliorate pulmonary fibrosis via the ATF3/PINK1 pathway.
Vascular Smooth Muscle FTO Promotes Aortic Dissecting Aneurysms via m6A Modification of Klf5.
GRHL2 Upregulation Predicts a Poor Prognosis and Promotes the Resistance of Serous Ovarian Cancer to Cisplatin.
The Effects of Puerarin on Autophagy Through Regulating of the PERK/eIF2α/ATF4 Signaling Pathway Influences Renal Function in Diabetic Nephropathy.
Methamphetamine induces GSDME-dependent cell death in hippocampal neuronal cells through the endoplasmic reticulum stress pathway.
Reactive oxygen species mediate anlotinib-induced apoptosis via activation of endoplasmic reticulum stress in pancreatic cancer.
Tectorigenin enhances PDX1 expression and protects pancreatic β-cells by activating ERK and reducing ER stress.
Melatonin inhibits oxalate-induced endoplasmic reticulum stress and apoptosis in HK-2 cells by activating the AMPK pathway.
Melatonin suppresses ER stress-dependent proapoptotic effects via AMPK in bone mesenchymal stem cells during mitochondrial oxidative damage.
Ginsenosides reduce body weight and ameliorate hepatic steatosis in high fat diet‑induced obese mice via endoplasmic reticulum stress and p‑STAT3/STAT3 signaling.
Hydrogen sulfide regulates muscle RING finger-1 protein S-sulfhydration at Cys(44) to prevent cardiac structural damage in diabetic cardiomyopathy.
Human cathelicidin antimicrobial peptide suppresses proliferation, migration and invasion of oral carcinoma HSC-3 cells via a novel mechanism involving caspase-3 mediated apoptosis.
Pseudorabies virus infection inhibits stress granules formation via dephosphorylating eIF2α.
Hydrogen sulphide ameliorating skeletal muscle atrophy in db/db mice via Muscle RING finger 1 S-sulfhydration.
Suhuang antitussive capsule inhibits NLRP3 inflammasome activation and ameliorates pulmonary dysfunction via suppression of endoplasmic reticulum stress in cough variant asthma.
Icariin improves the cognitive function of APP/PS1 mice via suppressing endoplasmic reticulum stress.
Protective effect of Barbaloin in a rat model of myocardial ischemia reperfusion injury through the regulation of the CNPY2‑PERK pathway.
Endoplasmic reticulum stress induces apoptosis of arginine vasopressin neurons in central diabetes insipidus via PI3K/Akt pathway.
Oxytocin Suppresses Inflammatory Responses Induced by Lipopolysaccharide through Inhibition of the eIF-2-ATF4 Pathway in Mouse Microglia.
Quantitative Proteomic Analysis Reveals Unfolded-Protein Response Involved in Severe Fever with Thrombocytopenia Syndrome Virus Infection.
Pterostilbene exerts a protective effect via regulating tunicamycin-induced endoplasmic reticulum stress in mouse preimplantation embryos.
KMT2D deficiency enhances the anti-cancer activity of L48H37 in pancreatic ductal adenocarcinoma.
Betulinic acid alleviates endoplasmic reticulum stress-mediated nonalcoholic fatty liver disease through activation of farnesoid X receptors in mice.
Differentially expressed genes of HepG2 cells treated with gecko polypeptide mixture.
Network pharmacology-based identification of major component of Angelica sinensis and its action mechanism for the treatment of acute myocardial infarction.
Protective effect of resveratrol against nigrostriatal pathway injury in striatum via JNK pathway.
Activating Transcription Factor 4 (ATF4)-ATF3-C/EBP Homologous Protein (CHOP) Cascade Shows an Essential Role in the ER Stress-Induced Sensitization of Tetrachlorobenzoquinone-Challenged PC12 Cells to ROS-Mediated Apoptosis via Death Receptor 5 (DR5) Signaling.
Rifampicin-induced injury in L02 cells is alleviated by 4-PBA via inhibition of the PERK-ATF4-CHOP pathway.
Alpha-lipoic acid defends homocysteine-induced endoplasmic reticulum and oxidative stress in HAECs.
Vinca alkaloid drugs promote stress-induced translational repression and stress granule formation.
Simvastatin exerts anti-hepatitis B virus activity by inhibiting expression of minichromosome maintenance protein 7 in HepG2.2.15 cells.
PACE4 regulates apoptosis in human prostate cancer cells via endoplasmic reticulum stress and mitochondrial signaling pathways.
Astragaloside IV attenuates proteinuria in streptozotocin-induced diabetic nephropathy via the inhibition of endoplasmic reticulum stress.
O-Alkylated derivatives of quercetin induce apoptosis of MCF-7 cells via a caspase-independent mitochondrial pathway.
Li F, Geng X, Lee H, Wills M, Ding Y
Frontiers in cellular neuroscience 2021;15:598230
Frontiers in cellular neuroscience 2021;15:598230
Interventional Efects of the Topical of "Sanse Powder" Essential Oils Nanoemulsion on Knee Osteoarthritis in Rats by Targeting the ERS/TXNIP/NLRP3 Signaling Axis.
Liu Z, Liao T, Yang N, Ding L, Li X, Wu P, Wang P
Frontiers in pharmacology 2021;12:739644
Frontiers in pharmacology 2021;12:739644
Kaempferol Alleviates Steatosis and Inflammation During Early Non-Alcoholic Steatohepatitis Associated With Liver X Receptor α-Lysophosphatidylcholine Acyltransferase 3 Signaling Pathway.
Xiang H, Shao M, Lu Y, Wang J, Wu T, Ji G
Frontiers in pharmacology 2021;12:690736
Frontiers in pharmacology 2021;12:690736
Inhibition of the miR-193b-3p protects against oxidized low-density lipoprotein-induced HUVECs injury by upregulating ALDH2.
Bai Y, Wang M, Yang Y, Liu X, Chen Q, Guo Z
Cell biology international 2021 Oct 31;
Cell biology international 2021 Oct 31;
Cytotoxic BODIPY-Appended Half-Sandwich Iridium(III) Complex Forms Protein Adducts and Induces ER Stress.
Ramos R, Gilles JF, Morichon R, Przybylski C, Caron B, Botuha C, Karaiskou A, Salmain M, Sobczak-Thépot J
Journal of medicinal chemistry 2021 Nov 25;64(22):16675-16686
Journal of medicinal chemistry 2021 Nov 25;64(22):16675-16686
Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy.
Vanhoutte D, Schips TG, Vo A, Grimes KM, Baldwin TA, Brody MJ, Accornero F, Sargent MA, Molkentin JD
Nature communications 2021 Jun 24;12(1):3928
Nature communications 2021 Jun 24;12(1):3928
Choline attenuates heat stress-induced oxidative injury and apoptosis in bovine mammary epithelial cells by modulating PERK/Nrf-2 signaling pathway.
Yang M, Kuang M, Wang G, Ali I, Tang Y, Yang C, Li Y, Li L
Molecular immunology 2021 Jul;135:388-397
Molecular immunology 2021 Jul;135:388-397
MCLR induces dysregulation of calcium homeostasis and endoplasmic reticulum stress resulting in apoptosis in Sertoli cells.
Zhao S, Yuan C, Tuo X, Zhou C, Zhao Q, Shen T
Chemosphere 2021 Jan;263:127868
Chemosphere 2021 Jan;263:127868
Microcystin-leucine arginine induced the apoptosis of GnRH neurons by activating the endoplasmic reticulum stress resulting in a decrease of serum testosterone level in mice.
Jin H, Hou J, Meng X, Ma T, Wang B, Liu Z, Sha X, Ding J, Han X
Ecotoxicology and environmental safety 2021 Jan 15;208:111748
Ecotoxicology and environmental safety 2021 Jan 15;208:111748
Endoplasmic reticulum stress pathway mediates T-2 toxin-induced chondrocyte apoptosis.
Liu YN, Mu YD, Wang H, Zhang M, Shi YW, Mi G, Peng LX, Chen JH
Toxicology 2021 Dec;464:152989
Toxicology 2021 Dec;464:152989
Deficiency of β-arrestin2 alleviates apoptosis through GRP78-ATF6-CHOP signaling pathway in primary Sjögren's syndrome.
Huang L, Liu Q, Zhou T, Zhang J, Tian Q, Zhang Q, Wei W, Wu H
International immunopharmacology 2021 Dec;101(Pt A):108281
International immunopharmacology 2021 Dec;101(Pt A):108281
Mannan-binding lectin deficiency augments hepatic endoplasmic reticulum stress through IP3R-controlled calcium release.
Chen Y, Hu M, Deng F, Wang P, Lin J, Zheng Z, Liu Y, Dong L, Lu X, Chen Z, Zhou J, Zuo D
Cell calcium 2021 Dec;100:102477
Cell calcium 2021 Dec;100:102477
Citrus alkaline extracts prevent endoplasmic reticulum stress in type II alveolar epithelial cells to ameliorate pulmonary fibrosis via the ATF3/PINK1 pathway.
Wang Z, Feng F, He H, Wu Q, Gu C, Hrovat J, Peng W, Xu Y, Han D, Yang P, Zhou X, Li J, Shan J
Phytomedicine : international journal of phytotherapy and phytopharmacology 2021 Aug;89:153599
Phytomedicine : international journal of phytotherapy and phytopharmacology 2021 Aug;89:153599
Vascular Smooth Muscle FTO Promotes Aortic Dissecting Aneurysms via m6A Modification of Klf5.
Ma D, Liu X, Zhang JJ, Zhao JJ, Xiong YJ, Chang Q, Wang HY, Su P, Meng J, Zhao YB
Frontiers in cardiovascular medicine 2020;7:592550
Frontiers in cardiovascular medicine 2020;7:592550
GRHL2 Upregulation Predicts a Poor Prognosis and Promotes the Resistance of Serous Ovarian Cancer to Cisplatin.
Nie Y, Ding Y, Yang M
OncoTargets and therapy 2020;13:6303-6314
OncoTargets and therapy 2020;13:6303-6314
The Effects of Puerarin on Autophagy Through Regulating of the PERK/eIF2α/ATF4 Signaling Pathway Influences Renal Function in Diabetic Nephropathy.
Xu X, Chen B, Huang Q, Wu Y, Liang T
Diabetes, metabolic syndrome and obesity : targets and therapy 2020;13:2583-2592
Diabetes, metabolic syndrome and obesity : targets and therapy 2020;13:2583-2592
Methamphetamine induces GSDME-dependent cell death in hippocampal neuronal cells through the endoplasmic reticulum stress pathway.
Liu Y, Wen D, Gao J, Xie B, Yu H, Shen Q, Zhang J, Jing W, Cong B, Ma C
Brain research bulletin 2020 Sep;162:73-83
Brain research bulletin 2020 Sep;162:73-83
Reactive oxygen species mediate anlotinib-induced apoptosis via activation of endoplasmic reticulum stress in pancreatic cancer.
Yang L, Zhou X, Sun J, Lei Q, Wang Q, Pan D, Ding M, Ding Y
Cell death & disease 2020 Sep 17;11(9):766
Cell death & disease 2020 Sep 17;11(9):766
Tectorigenin enhances PDX1 expression and protects pancreatic β-cells by activating ERK and reducing ER stress.
Yao X, Li K, Liang C, Zhou Z, Wang J, Wang S, Liu L, Yu CL, Song ZB, Bao YL, Zheng LH, Sun Y, Wang G, Huang Y, Yi J, Sun L, Li Y
The Journal of biological chemistry 2020 Sep 11;295(37):12975-12992
The Journal of biological chemistry 2020 Sep 11;295(37):12975-12992
Melatonin inhibits oxalate-induced endoplasmic reticulum stress and apoptosis in HK-2 cells by activating the AMPK pathway.
Song Q, He Z, Li B, Liu J, Liu L, Liao W, Xiong Y, Song C, Yang S, Liu Y
Cell cycle (Georgetown, Tex.) 2020 Oct;19(20):2600-2610
Cell cycle (Georgetown, Tex.) 2020 Oct;19(20):2600-2610
Melatonin suppresses ER stress-dependent proapoptotic effects via AMPK in bone mesenchymal stem cells during mitochondrial oxidative damage.
Fan C, Feng J, Tang C, Zhang Z, Feng Y, Duan W, Zhai M, Yan Z, Zhu L, Feng L, Zhu H, Luo E
Stem cell research & therapy 2020 Oct 15;11(1):442
Stem cell research & therapy 2020 Oct 15;11(1):442
Ginsenosides reduce body weight and ameliorate hepatic steatosis in high fat diet‑induced obese mice via endoplasmic reticulum stress and p‑STAT3/STAT3 signaling.
Yao Y
Molecular medicine reports 2020 Mar;21(3):1059-1070
Molecular medicine reports 2020 Mar;21(3):1059-1070
Hydrogen sulfide regulates muscle RING finger-1 protein S-sulfhydration at Cys(44) to prevent cardiac structural damage in diabetic cardiomyopathy.
Sun X, Zhao D, Lu F, Peng S, Yu M, Liu N, Sun Y, Du H, Wang B, Chen J, Dong S, Lu F, Zhang W
British journal of pharmacology 2020 Feb;177(4):836-856
British journal of pharmacology 2020 Feb;177(4):836-856
Human cathelicidin antimicrobial peptide suppresses proliferation, migration and invasion of oral carcinoma HSC-3 cells via a novel mechanism involving caspase-3 mediated apoptosis.
Chen X, Ji S, Si J, Zhang X, Wang X, Guo Y, Zou X
Molecular medicine reports 2020 Dec;22(6):5243-5250
Molecular medicine reports 2020 Dec;22(6):5243-5250
Pseudorabies virus infection inhibits stress granules formation via dephosphorylating eIF2α.
Xu S, Chen D, Chen D, Hu Q, Zhou L, Ge X, Han J, Guo X, Yang H
Veterinary microbiology 2020 Aug;247:108786
Veterinary microbiology 2020 Aug;247:108786
Hydrogen sulphide ameliorating skeletal muscle atrophy in db/db mice via Muscle RING finger 1 S-sulfhydration.
Lu F, Lu B, Zhang L, Wen J, Wang M, Zhang S, Li Q, Shu F, Sun Y, Liu N, Peng S, Zhao Y, Dong S, Zhao D, Lu F, Zhang W
Journal of cellular and molecular medicine 2020 Aug;24(16):9362-9377
Journal of cellular and molecular medicine 2020 Aug;24(16):9362-9377
Suhuang antitussive capsule inhibits NLRP3 inflammasome activation and ameliorates pulmonary dysfunction via suppression of endoplasmic reticulum stress in cough variant asthma.
Qin W, Wu X, Jia Y, Tong X, Guo C, Chen D, Wang Z, Tan N
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Oct;118:109188
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Oct;118:109188
Icariin improves the cognitive function of APP/PS1 mice via suppressing endoplasmic reticulum stress.
Li F, Zhang Y, Lu X, Shi J, Gong Q
Life sciences 2019 Oct 1;234:116739
Life sciences 2019 Oct 1;234:116739
Protective effect of Barbaloin in a rat model of myocardial ischemia reperfusion injury through the regulation of the CNPY2‑PERK pathway.
Cui Y, Wang Y, Liu G
International journal of molecular medicine 2019 May;43(5):2015-2023
International journal of molecular medicine 2019 May;43(5):2015-2023
Endoplasmic reticulum stress induces apoptosis of arginine vasopressin neurons in central diabetes insipidus via PI3K/Akt pathway.
Zhou MF, Feng ZP, Ou YC, Peng JJ, Li K, Gong HD, Qiu BH, Liu YW, Wang YJ, Qi ST
CNS neuroscience & therapeutics 2019 May;25(5):562-574
CNS neuroscience & therapeutics 2019 May;25(5):562-574
Oxytocin Suppresses Inflammatory Responses Induced by Lipopolysaccharide through Inhibition of the eIF-2-ATF4 Pathway in Mouse Microglia.
Inoue T, Yamakage H, Tanaka M, Kusakabe T, Shimatsu A, Satoh-Asahara N
Cells 2019 May 31;8(6)
Cells 2019 May 31;8(6)
Quantitative Proteomic Analysis Reveals Unfolded-Protein Response Involved in Severe Fever with Thrombocytopenia Syndrome Virus Infection.
Zhang LK, Wang B, Xin Q, Shang W, Shen S, Xiao G, Deng F, Wang H, Hu Z, Wang M
Journal of virology 2019 May 15;93(10)
Journal of virology 2019 May 15;93(10)
Pterostilbene exerts a protective effect via regulating tunicamycin-induced endoplasmic reticulum stress in mouse preimplantation embryos.
Ullah O, Li Z, Ali I, Xu L, Liu H, Jin HZ, Fang YY, Jin QG, Fang N
In vitro cellular & developmental biology. Animal 2019 Feb;55(2):82-93
In vitro cellular & developmental biology. Animal 2019 Feb;55(2):82-93
KMT2D deficiency enhances the anti-cancer activity of L48H37 in pancreatic ductal adenocarcinoma.
Li SS, Jiang WL, Xiao WQ, Li K, Zhang YF, Guo XY, Dai YQ, Zhao QY, Jiang MJ, Lu ZJ, Wan R
World journal of gastrointestinal oncology 2019 Aug 15;11(8):599-621
World journal of gastrointestinal oncology 2019 Aug 15;11(8):599-621
Betulinic acid alleviates endoplasmic reticulum stress-mediated nonalcoholic fatty liver disease through activation of farnesoid X receptors in mice.
Gu M, Zhao P, Zhang S, Fan S, Yang L, Tong Q, Ji G, Huang C
British journal of pharmacology 2019 Apr;176(7):847-863
British journal of pharmacology 2019 Apr;176(7):847-863
Differentially expressed genes of HepG2 cells treated with gecko polypeptide mixture.
Duan YM, Jin Y, Guo ML, Duan LX, Wang JG
Journal of Cancer 2018;9(15):2723-2733
Journal of Cancer 2018;9(15):2723-2733
Network pharmacology-based identification of major component of Angelica sinensis and its action mechanism for the treatment of acute myocardial infarction.
Niu X, Zhang J, Ni J, Wang R, Zhang W, Sun S, Peng Y, Bai M, Zhang Z
Bioscience reports 2018 Dec 21;38(6)
Bioscience reports 2018 Dec 21;38(6)
Protective effect of resveratrol against nigrostriatal pathway injury in striatum via JNK pathway.
Li D, Liu N, Zhao L, Tong L, Kawano H, Yan HJ, Li HP
Brain research 2017 Jan 1;1654(Pt A):1-8
Brain research 2017 Jan 1;1654(Pt A):1-8
Activating Transcription Factor 4 (ATF4)-ATF3-C/EBP Homologous Protein (CHOP) Cascade Shows an Essential Role in the ER Stress-Induced Sensitization of Tetrachlorobenzoquinone-Challenged PC12 Cells to ROS-Mediated Apoptosis via Death Receptor 5 (DR5) Signaling.
Liu Z, Shi Q, Song X, Wang Y, Wang Y, Song E, Song Y
Chemical research in toxicology 2016 Sep 19;29(9):1510-8
Chemical research in toxicology 2016 Sep 19;29(9):1510-8
Rifampicin-induced injury in L02 cells is alleviated by 4-PBA via inhibition of the PERK-ATF4-CHOP pathway.
Zhang W, Chen L, Shen Y, Xu J
Toxicology in vitro : an international journal published in association with BIBRA 2016 Oct;36:186-196
Toxicology in vitro : an international journal published in association with BIBRA 2016 Oct;36:186-196
Alpha-lipoic acid defends homocysteine-induced endoplasmic reticulum and oxidative stress in HAECs.
Hu H, Wang C, Jin Y, Meng Q, Liu Q, Liu K, Sun H
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2016 May;80:63-72
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2016 May;80:63-72
Vinca alkaloid drugs promote stress-induced translational repression and stress granule formation.
Szaflarski W, Fay MM, Kedersha N, Zabel M, Anderson P, Ivanov P
Oncotarget 2016 May 24;7(21):30307-22
Oncotarget 2016 May 24;7(21):30307-22
Simvastatin exerts anti-hepatitis B virus activity by inhibiting expression of minichromosome maintenance protein 7 in HepG2.2.15 cells.
Li W, Cao F, Li J, Wang Z, Ren Y, Liang Z, Liu P
Molecular medicine reports 2016 Dec;14(6):5334-5342
Molecular medicine reports 2016 Dec;14(6):5334-5342
PACE4 regulates apoptosis in human prostate cancer cells via endoplasmic reticulum stress and mitochondrial signaling pathways.
Yao Z, Sun B, Hong Q, Yan J, Mu D, Li J, Sheng H, Guo H
Drug design, development and therapy 2015;9:5911-23
Drug design, development and therapy 2015;9:5911-23
Astragaloside IV attenuates proteinuria in streptozotocin-induced diabetic nephropathy via the inhibition of endoplasmic reticulum stress.
Wang ZS, Xiong F, Xie XH, Chen D, Pan JH, Cheng L
BMC nephrology 2015 Mar 31;16:44
BMC nephrology 2015 Mar 31;16:44
O-Alkylated derivatives of quercetin induce apoptosis of MCF-7 cells via a caspase-independent mitochondrial pathway.
Liao H, Bao X, Zhu J, Qu J, Sun Y, Ma X, Wang E, Guo X, Kang Q, Zhen Y
Chemico-biological interactions 2015 Dec 5;242:91-8
Chemico-biological interactions 2015 Dec 5;242:91-8
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 20582-1-AP(PERK antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The PERK antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human PERK. This antibody recognizes human antigen. The PERK antibody has been validated for the following applications: ELISA, WB, IHC analysis.
