bs-3330R

antibody from Bioss

Targeting: EIF2AK3 PEK, PERK

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

bs-3330R

Provider

Bioss

Proper citation

Bioss Cat#bs-3330R, RRID:AB_10855345

Product name

Rabbit Anti-PERK(Thr980) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

PERK Thr980

Description

Purified by Protein A.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100ul

Concentration

1ug/ul

Storage

Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

References

Submitted references

Gestational exposure to PM_2.5 impairs vascularization of the placenta.

Yue H, Ji X, Zhang Y, Li G, Sang N
The Science of the total environment 2019 May 15;665:153-161

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Flow-enhanced vascularization and maturation of kidney organoids in vitro.

Homan KA, Gupta N, Kroll KT, Kolesky DB, Skylar-Scott M, Miyoshi T, Mau D, Valerius MT, Ferrante T, Bonventre JV, Lewis JA, Morizane R
Nature methods 2019 Mar;16(3):255-262

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Hepatoprotective effects of limb ischemic post-conditioning in hepatic ischemic rat model and liver cancer patients via PI3K/ERK pathways.

Gao Y, Zhou S, Wang F, Zhou Y, Sheng S, Qi D, Huang JH, Wu E, Lv Y, Huo X
International journal of biological sciences 2018;14(14):2037-2050

---

miR-103 promotes the metastasis and EMT of hepatocellular carcinoma by directly inhibiting LATS2.

Han LL, Yin XR, Zhang SQ
International journal of oncology 2018 Dec;53(6):2433-2444

---

Expression of Phosphorylated KIT in Canine Mast Cell Tumor.

Halsey CHC, Thamm DH, Weishaar KM, Burton JH, Charles JB, Gustafson DL, Avery AC, Ehrhart EJ
Veterinary pathology 2017 May;54(3):387-394

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Cardiac Contractility Modulation Attenuate Myocardial Fibrosis by Inhibiting TGF-β1/Smad3 Signaling Pathway in a Rabbit Model of Chronic Heart Failure.

Zhang F, Dang Y, Li Y, Hao Q, Li R, Qi X
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2016;39(1):294-302

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CEACAM1 mediates B cell aggregation in central nervous system autoimmunity.

Rovituso DM, Scheffler L, Wunsch M, Kleinschnitz C, Dörck S, Ulzheimer J, Bayas A, Steinman L, Ergün S, Kuerten S
Scientific reports 2016 Jul 20;6:29847

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Huangqin-tang ameliorates dextran sodium sulphate-induced colitis by regulating intestinal epithelial cell homeostasis, inflammation and immune response.

Zou Y, Lin J, Li W, Wu Z, He Z, Huang G, Wang J, Ye C, Cheng X, Ding C, Zheng X, Chi H
Scientific reports 2016 Dec 16;6:39299

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Blockade of hypoxia-induced CXCR4 with AMD3100 inhibits production of OA-associated catabolic mediators IL-1β and MMP-13.

Li P, Deng J, Wei X, Jayasuriya CT, Zhou J, Chen Q, Zhang J, Wei L, Wei F
Molecular medicine reports 2016 Aug;14(2):1475-82

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Targeting the Wnt-Regulatory Protein CTNNBIP1 by microRNA-214 Enhances the Stemness and Self-Renewal of Cancer Stem-Like Cells in Lung Adenocarcinomas.

Qi W, Chen J, Cheng X, Huang J, Xiang T, Li Q, Long H, Zhu B
Stem cells (Dayton, Ohio) 2015 Dec;33(12):3423-36

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The effect of electroacupuncture on the extracellular matrix synthesis and degradation in a rabbit model of disc degeneration.

Huang GF, Zou J, Shi J, Zhang DY, Pen HF, Zhang Q, Gao Y, Wang BY
Evidence-based complementary and alternative medicine : eCAM 2014;2014:731395

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Inhibition of neutral sphingomyelinase decreases elevated levels of inducible nitric oxide synthase and apoptotic cell death in ocular hypertensive rats.

Aslan M, Basaranlar G, Unal M, Ciftcioglu A, Derin N, Mutus B
Toxicology and applied pharmacology 2014 Nov 1;280(3):389-98

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Expression of selected genes involved in steroidogenesis in the course of enucleation-induced rat adrenal regeneration.

Tyczewska M, Rucinski M, Ziolkowska A, Trejter M, Szyszka M, Malendowicz LK
International journal of molecular medicine 2014 Mar;33(3):613-23

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Neutral sphingomyelinase inhibition decreases ER stress-mediated apoptosis and inducible nitric oxide synthase in retinal pigment epithelial cells.

Kucuksayan E, Konuk EK, Demir N, Mutus B, Aslan M
Free radical biology & medicine 2014 Jul;72:113-23

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Sustained endoplasmic reticulum stress inhibits hepatocyte proliferation via downregulation of c-Met expression.

He Y, Long J, Zhong W, Fu Y, Li Y, Lin S
Molecular and cellular biochemistry 2014 Apr;389(1-2):151-8

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Chemoprevention activity of dipyridamole in the MMTV-PyMT transgenic mouse model of breast cancer.

Wang C, Schwab LP, Fan M, Seagroves TN, Buolamwini JK
Cancer prevention research (Philadelphia, Pa.) 2013 May;6(5):437-47

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RPB5-mediating protein is required for the proliferation of hepatocellular carcinoma cells.

Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W
The Journal of biological chemistry 2011 Apr 1;286(13):11865-74

Immunohistochemistry

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-PERK(Thr980) Polyclonal Antibody, Unconjugated (bs-3330R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Sample type

Rat

Other comments

Brain

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human lung carcinoma labeled with Anti-Phospho-PERK(Thr980) Polyclonal Antibody, Unconjugated(bs-3330R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.

Sample type

Human

Other comments

Lung