HPA015737

antibody from Atlas Antibodies

Targeting: EIF2AK3 PEK, PERK

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA015737

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA015737, RRID:AB_1848062

Product name

Anti-EIF2AK3

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

NAWLEAPPEKWQEKMDEIWLKDESTDWPLSSPSPM
DAPSVKIRRMDPFATKEHIEIIAPSPQRSRSFSVG
ISCDQTSSSESQFSPLEFSGMDHEDISESVDAAYN
LQDSCLTDCDVEDGTMDGNDE


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Submitted references

PARP16 is a tail-anchored endoplasmic reticulum protein required for the PERK- and IRE1α-mediated unfolded protein response

Jwa M, Chang P
Nature Cell Biology 2012 October;14(11):1223-1230

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human parathyroid gland and liver tissues using Anti-EIF2AK3 antibody. Corresponding EIF2AK3 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human parathyroid gland shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows low expression as expected.

Sample type

HUMAN