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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- NB100-68216 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-68216, RRID:AB_1107323
- Product name
- Rabbit Polyclonal Anillin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Anillin Antibody [NB100-68216] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-68216 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Anillin was also immunoprecipitated by NB100-68215, which recognizes an upstream epitope.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Anillin Antibody [NB100-68216] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Anillin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit .
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Anillin Antibody [NB100-68216] - FFPE section of human colon carcinoma. Affinity purified rabbit anti-Anillin used at a dilution of 1:250.
