Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NB100-1314 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1314, RRID:AB_788007
- Product name
- Goat Polyclonal Anillin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Functional analysis of a human homologue of the Drosophila actin binding protein anillin suggests a role in cytokinesis.
Functional analysis of a human homologue of the Drosophila actin binding protein anillin suggests a role in cytokinesis.
Oegema K, Savoian MS, Mitchison TJ, Field CM
The Journal of cell biology 2000 Aug 7;150(3):539-52
The Journal of cell biology 2000 Aug 7;150(3):539-52
Functional analysis of a human homologue of the Drosophila actin binding protein anillin suggests a role in cytokinesis.
Oegema K, Savoian MS, Mitchison TJ, Field CM
The Journal of cell biology 2000 Aug 7;150(3):539-52
The Journal of cell biology 2000 Aug 7;150(3):539-52
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Anillin Antibody [NB100-1314] - 10ug/ml staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Anillin Antibody [NB100-1314] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.