653708

antibody from BioLegend

Targeting: POU5F1 MGC22487, OCT3, Oct4, OTF3

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

653708

Provider

BioLegend

Proper citation

BioLegend Cat#653708, RRID:AB_2563184

Product name

Alexa Fluor® 594 anti-Oct4 (Oct3)

Antibody type

Monoclonal

Antigen

Partial human Oct4 recombinant protein (1-141aa).

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

3A2A20

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunocytochemistry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

NTERA-2 cl.D1 human embryoid carcinoma cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/ml anti-human Oct4 (Oct3) (clone 3A2A20) Alexa Fluor® 594 (red) in blocking buffer overnight at 4°C and followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.

Conjugate

Yellow dye