502927

antibody from BioLegend

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

502927

Provider

BioLegend

Proper citation

BioLegend Cat#502927, RRID:AB_2561314

Product name

Alexa Fluor® 700 anti-human TNF-a

Antibody type

Monoclonal

Antigen

E. coli-expressed, recombinant human TNF-α

Reactivity

Human, Porcine

Host

Mouse

Conjugate

Near infrared dye

Isotype

IgG

Antibody clone number

MAb11

Vial size

25 tests

Storage

4° C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes (in the presence of monensin) were stained with CD3 PE, then fixed, permeabilized and stained with TNF-α (clone MAb11) Alexa Fluor® 700 (top). The bottom dot plot shows the staining can by blocked by pre-incubation with unlabeled purified MAb11 antibody.

Conjugate

Near infrared dye