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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- NBP2-27224 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TNF-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TNF-alpha Antibody (6N1E7) - Azide Free [NBP2-27224] - Analysis of TNF alpha recombinant protein using TNF alpha antibody at 3 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TNF-alpha Antibody (6N1E7) - Azide Free [NBP2-27224] - Human PBMC were stimulated with PMA (10 ng/ml) and Ionomycin (500 ng/ml) for 5h in presence of Brefeldin A. At the end of stimulation, cells were surface stained with before testing with by intracellular staining. Fixed and permeabilized cells were incubated with 10 ul (0.5 ug) of TNF antibody (or ) for 30 minutes on ice. Lymphocyte population was gated for analysis to show TNF alpha positive T cells.
