Antibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Other assay [1]
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- Product number
- M2TNFAI - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TNF alpha Monoclonal Antibody (E10042)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- M2TNFAI targets TNF Alpha in ELISA applications and shows reactivity with Human samples.
- Antibody clone number
- E10042
- Concentration
- 1 mg/mL
Submitted references Enhanced interleukin-12 and CD40 ligand activities but reduced Staphylococcus aureus Cowan 1-induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis.
Enhanced interleukin-12 and CD40 ligand activities but reduced Staphylococcus aureus Cowan 1-induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis.
Human dendritic cells are activated by dengue virus infection: enhancement by gamma interferon and implications for disease pathogenesis.
Generation and cytotoxic profile of human peripheral blood CD4+ T lymphocytes.
Montenegro SM, Abath FG, Domingues AL, Melo WG, Morais CN, Coutinho EM, Mahanty S, Wynn TA
Infection and immunity 2002 Nov;70(11):5903-12
Infection and immunity 2002 Nov;70(11):5903-12
Enhanced interleukin-12 and CD40 ligand activities but reduced Staphylococcus aureus Cowan 1-induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis.
Montenegro SM, Abath FG, Domingues AL, Melo WG, Morais CN, Coutinho EM, Mahanty S, Wynn TA
Infection and immunity 2002 Nov;70(11):5903-12
Infection and immunity 2002 Nov;70(11):5903-12
Human dendritic cells are activated by dengue virus infection: enhancement by gamma interferon and implications for disease pathogenesis.
Libraty DH, Pichyangkul S, Ajariyakhajorn C, Endy TP, Ennis FA
Journal of virology 2001 Apr;75(8):3501-8
Journal of virology 2001 Apr;75(8):3501-8
Generation and cytotoxic profile of human peripheral blood CD4+ T lymphocytes.
Smyth MJ
Immunology and cell biology 1992 Dec;70 ( Pt 6):379-90
Immunology and cell biology 1992 Dec;70 ( Pt 6):379-90
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Supportive validation
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- Experimental details
- Direct ELISA analysis of TNF alpha was performed by coating wells of a 96-well plate with 100 µL per well of human recombinant protein TNF alpha (Product # RTNFAI) diluted in carbonate/bicarbonate buffer (Product # 28382) at a concentration of 1 µg/mL overnight at 4C. Wells of the plate were washed, blocked with starting blocking buffer (Product # 37538), and incubated with 100 µL per well of a mouse anti-TNF alpha monoclonal antibody (Product # M2TNFAI) at a dilution of 1:25, 1:50, 1:100, 1:200, 1:400, 1:800, 1:1600 and 1:3200 for 90 minutes at 37C. The plate was washed, then incubated with 100 µL per well of a HRP-conjugated goat anti-mouse IgG secondary antibody (Product # 62-6520) at a dilution of 1:2000 for 90 minutes at 37C. Detection was performed using 1-Step Ultra TMB substrate (Product # 34028) for 5-10 minutes at room temperature in the dark. The reaction was stopped with Stop solution (Product # N600), and absorbances were read on a spectrophotometer at 450-550 nm.