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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Product number
- IC210F - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TNF-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects cytoplasmic forms of human TNF-alpha .
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Monocyte Differentiation towards Protumor Activity Does Not Correlate with M1 or M2 Phenotypes.
Expression of the T cell receptor αβ on a CD123+ BDCA2+ HLA-DR+ subpopulation in head and neck squamous cell carcinoma.
Kinin receptor expression during Staphylococcus aureus infection.
Nucleoporation of dendritic cells: efficient gene transfer by electroporation into human monocyte-derived dendritic cells.
Nucleoporation of dendritic cells: efficient gene transfer by electroporation into human monocyte-derived dendritic cells.
Chimal-Ramírez GK, Espinoza-Sánchez NA, Chávez-Sánchez L, Arriaga-Pizano L, Fuentes-Pananá EM
Journal of immunology research 2016;2016:6031486
Journal of immunology research 2016;2016:6031486
Expression of the T cell receptor αβ on a CD123+ BDCA2+ HLA-DR+ subpopulation in head and neck squamous cell carcinoma.
Thiel A, Kesselring R, Pries R, Puzik A, Wittkopf N, Wollenberg B
PloS one 2011 Jan 11;6(1):e15997
PloS one 2011 Jan 11;6(1):e15997
Kinin receptor expression during Staphylococcus aureus infection.
Bengtson SH, Phagoo SB, Norrby-Teglund A, Påhlman L, Mörgelin M, Zuraw BL, Leeb-Lundberg LM, Herwald H
Blood 2006 Sep 15;108(6):2055-63
Blood 2006 Sep 15;108(6):2055-63
Nucleoporation of dendritic cells: efficient gene transfer by electroporation into human monocyte-derived dendritic cells.
Lenz P, Bacot SM, Frazier-Jessen MR, Feldman GM
FEBS letters 2003 Mar 13;538(1-3):149-54
FEBS letters 2003 Mar 13;538(1-3):149-54
Nucleoporation of dendritic cells: efficient gene transfer by electroporation into human monocyte-derived dendritic cells.
Lenz P, Bacot SM, Frazier-Jessen MR, Feldman GM
FEBS letters 2003 Mar 13;538(1-3):149-54
FEBS letters 2003 Mar 13;538(1-3):149-54
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of TNF-alpha in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA and Ca2+ ionomycin for 24 hours were stained with Mouse Anti-Human TNF-alpha Fluorescein-conjugated Monoclonal Antibody (Catalog # IC210F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of TNF-alpha in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA and Ca2+ ionomycin for 3.5 hours (filled histogram) or untreated (open histogram) were stained with Mouse Anti-Human TNF-alpha Fluorescein-conjugated Monoclonal Antibody (Catalog # IC210F) or isotype control antibody (Catalog # IC002F, not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of TNF- alpha in Human PBMCs by Flow Cytometry.
