AF1011

antibody from Novus Biologicals

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1011

Provider

Novus Biologicals

Product name

Goat Polyclonal TNF-alpha Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects cotton rat TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, greater than 50% cross-reactivity with recombinant mouse TNF-alpha is observed, approximately 30% cross-reactivity with recombinant rat TNF-alpha is observed, 20% cross-reactivity with recombinant human TNF-alpha is observed, and 5% cross-reactivity with recombinant porcine TNF-alpha is observed.

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cytotoxicity Induced by TNF-alpha and Neutralization by Cotton Rat TNF-alpha Antibody. Recombinant Cotton Rat TNF-alpha (Catalog # 1011-CR) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Cotton Rat TNF-alpha (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1011). The ND₅₀ is typically 0.01-0.05 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).