MAB510-100

antibody from Novus Biologicals

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB510-100

Provider

Novus Biologicals

Product name

Mouse Monoclonal TNF-alpha Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects rat TNF-alpha in ELISAs and Western blots. In ELISAs, this antibody shows less than 3% cross-reactivity with recombinant mouse (rm) TNF-alpha and less than 0.2% cross-reactivity with rhTNF-alpha , rpTNF-alpha , and rhTNF-beta . In Western blots, this antibody shows 100% cross-reactivity with rmTNF-alpha and no cross-reactivity with rfeTNF-alpha .

Reactivity

Rat

Host

Mouse

Isotype

IgG

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cytotoxicity Induced by TNF-alpha and Neutralization by Rat TNF-alpha Antibody. Recombinant Rat TNF-alpha (Catalog # 510-RT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Rat TNF-alpha (0.025 ng/mL) is neutralized (green line) by increasing concentrations of Rat TNF-alpha Monoclonal Antibody (Catalog # MAB510). The ND₅₀ is typically 10-40 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).