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- Antibody Data
- Antigen structure
- References [35]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB610-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TNF-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TNF-alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human TNF-beta , recombinant mouse TNF-alpha , recombinant rat TNF-alpha , and recombinant porcine TNF-alpha is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P01375- Isotype
- IgG
- Antibody clone number
- 28401
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Dysfunctional endothelial-derived microparticles promote inflammatory macrophage formation via NF-кB and IL-1β signal pathways.
The induced RNA-binding protein, HuR, targets 3'-UTR region of IL-6 mRNA and enhances its stabilization in periodontitis.
Differential expression profile of CXCR3 splicing variants is associated with thyroid neoplasia. Potential role in papillary thyroid carcinoma oncogenesis?
B cells inhibit bone formation in rheumatoid arthritis by suppressing osteoblast differentiation.
Effects of anti-inflammatory drugs on the expression of tryptophan-metabolism genes by human macrophages.
Combination of IAP antagonist and IFNγ activates novel caspase-10- and RIPK1-dependent cell death pathways.
Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation.
Lysine fatty acylation promotes lysosomal targeting of TNF-α.
Elevated Membrane and Soluble CD64: A Novel Marker Reflecting Altered FcγR Function and Disease in Early Rheumatoid Arthritis That Can Be Regulated by Anti-Rheumatic Treatment.
Increased CD160 expression on circulating natural killer cells in atherogenesis.
Macrophages and dendritic cells as actors in the immune reaction of classical Hodgkin lymphoma.
Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
The novel SMAC mimetic birinapant exhibits potent activity against human melanoma cells.
A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment.
C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
Protection from RNA and DNA viruses by IL-32.
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice.
Tumor necrosis factor alpha as a new target for renal cell carcinoma: two sequential phase II trials of infliximab at standard and high dose.
Ultrasensitive flow-based immunoassays using single-molecule counting.
Lymphocytes and synovial fluid fibroblasts support osteoclastogenesis through RANKL, TNFalpha, and IL-7 in an in vitro model derived from human psoriatic arthritis.
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Interleukin-10 modulates pro-apoptotic effects of TNF-alpha in human articular chondrocytes in vitro.
Increased ENA-78 in the follicular fluid of patients with endometriosis.
Sequential induction of angiogenic growth factors by TNF-alpha in choroidal endothelial cells.
Selective regulation of IL-10 signaling and function by zymosan.
True infliximab resistance in rheumatoid arthritis: a role for lymphotoxin alpha?
IFN-alpha priming results in a gain of proinflammatory function by IL-10: implications for systemic lupus erythematosus pathogenesis.
Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
trans fatty acids and systemic inflammation in heart failure.
Reprogramming of IL-10 activity and signaling by IFN-gamma.
Wang Y, Liu J, Chen X, Sun H, Peng S, Kuang Y, Pi J, Zhuang T, Zhang L, Yu Z, Tomlinson B, Chan P, Chen Y, Zhang Y, Li Y
Journal of cellular and molecular medicine 2019 Jan;23(1):476-486
Journal of cellular and molecular medicine 2019 Jan;23(1):476-486
The induced RNA-binding protein, HuR, targets 3'-UTR region of IL-6 mRNA and enhances its stabilization in periodontitis.
Ouhara K, Munenaga S, Kajiya M, Takeda K, Matsuda S, Sato Y, Hamamoto Y, Iwata T, Yamasaki S, Akutagawa K, Mizuno N, Fujita T, Sugiyama E, Kurihara H
Clinical and experimental immunology 2018 Jun;192(3):325-336
Clinical and experimental immunology 2018 Jun;192(3):325-336
Differential expression profile of CXCR3 splicing variants is associated with thyroid neoplasia. Potential role in papillary thyroid carcinoma oncogenesis?
Urra S, Fischer MC, Martínez JR, Véliz L, Orellana P, Solar A, Bohmwald K, Kalergis A, Riedel C, Corvalán AH, Roa JC, Fuentealba R, Cáceres CJ, López-Lastra M, León A, Droppelmann N, González HE
Oncotarget 2018 Jan 5;9(2):2445-2467
Oncotarget 2018 Jan 5;9(2):2445-2467
B cells inhibit bone formation in rheumatoid arthritis by suppressing osteoblast differentiation.
Sun W, Meednu N, Rosenberg A, Rangel-Moreno J, Wang V, Glanzman J, Owen T, Zhou X, Zhang H, Boyce BF, Anolik JH, Xing L
Nature communications 2018 Dec 3;9(1):5127
Nature communications 2018 Dec 3;9(1):5127
Effects of anti-inflammatory drugs on the expression of tryptophan-metabolism genes by human macrophages.
Regan T, Gill AC, Clohisey SM, Barnett MW, Pariante CM, Harrison NA, MRC Immunopsychiatry Consortium., Hume DA, Bullmore ET, Freeman TC
Journal of leukocyte biology 2018 Apr;103(4):681-692
Journal of leukocyte biology 2018 Apr;103(4):681-692
Combination of IAP antagonist and IFNγ activates novel caspase-10- and RIPK1-dependent cell death pathways.
Tanzer MC, Khan N, Rickard JA, Etemadi N, Lalaoui N, Spall SK, Hildebrand JM, Segal D, Miasari M, Chau D, Wong WL, McKinlay M, Chunduru SK, Benetatos CA, Condon SM, Vince JE, Herold MJ, Silke J
Cell death and differentiation 2017 Mar;24(3):481-491
Cell death and differentiation 2017 Mar;24(3):481-491
Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation.
Henry CM, Martin SJ
Molecular cell 2017 Feb 16;65(4):715-729.e5
Molecular cell 2017 Feb 16;65(4):715-729.e5
Lysine fatty acylation promotes lysosomal targeting of TNF-α.
Jiang H, Zhang X, Lin H
Scientific reports 2016 Apr 15;6:24371
Scientific reports 2016 Apr 15;6:24371
Elevated Membrane and Soluble CD64: A Novel Marker Reflecting Altered FcγR Function and Disease in Early Rheumatoid Arthritis That Can Be Regulated by Anti-Rheumatic Treatment.
Matt P, Lindqvist U, Kleinau S
PloS one 2015;10(9):e0137474
PloS one 2015;10(9):e0137474
Increased CD160 expression on circulating natural killer cells in atherogenesis.
Zuo J, Shan Z, Zhou L, Yu J, Liu X, Gao Y
Journal of translational medicine 2015 Jun 13;13:188
Journal of translational medicine 2015 Jun 13;13:188
Macrophages and dendritic cells as actors in the immune reaction of classical Hodgkin lymphoma.
Tudor CS, Bruns H, Daniel C, Distel LV, Hartmann A, Gerbitz A, Buettner MJ
PloS one 2014;9(12):e114345
PloS one 2014;9(12):e114345
Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
Todd J, Simpson P, Estis J, Torres V, Wub AH
Cytokine 2013 Dec;64(3):660-5
Cytokine 2013 Dec;64(3):660-5
The novel SMAC mimetic birinapant exhibits potent activity against human melanoma cells.
Krepler C, Chunduru SK, Halloran MB, He X, Xiao M, Vultur A, Villanueva J, Mitsuuchi Y, Neiman EM, Benetatos C, Nathanson KL, Amaravadi RK, Pehamberger H, McKinlay M, Herlyn M
Clinical cancer research : an official journal of the American Association for Cancer Research 2013 Apr 1;19(7):1784-94
Clinical cancer research : an official journal of the American Association for Cancer Research 2013 Apr 1;19(7):1784-94
A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment.
Kulbe H, Chakravarty P, Leinster DA, Charles KA, Kwong J, Thompson RG, Coward JI, Schioppa T, Robinson SC, Gallagher WM, Galletta L, Australian Ovarian Cancer Study Group., Salako MA, Smyth JF, Hagemann T, Brennan DJ, Bowtell DD, Balkwill FR
Cancer research 2012 Jan 1;72(1):66-75
Cancer research 2012 Jan 1;72(1):66-75
C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY
Diabetologia 2011 Oct;54(10):2713-23
Diabetologia 2011 Oct;54(10):2713-23
Protection from RNA and DNA viruses by IL-32.
Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4110-8
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4110-8
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation 2009 Jun 27;87(12):1814-20
Transplantation 2009 Jun 27;87(12):1814-20
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS research and human retroviruses 2008 Mar;24(3):453-62
AIDS research and human retroviruses 2008 Mar;24(3):453-62
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Gonzalez RM, Seurynck-Servoss SL, Crowley SA, Brown M, Omenn GS, Hayes DF, Zangar RC
Journal of proteome research 2008 Jun;7(6):2406-14
Journal of proteome research 2008 Jun;7(6):2406-14
Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice.
Kim HG, Kim NR, Gim MG, Lee JM, Lee SY, Ko MY, Kim JY, Han SH, Chung DK
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2553-61
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2553-61
Tumor necrosis factor alpha as a new target for renal cell carcinoma: two sequential phase II trials of infliximab at standard and high dose.
Harrison ML, Obermueller E, Maisey NR, Hoare S, Edmonds K, Li NF, Chao D, Hall K, Lee C, Timotheadou E, Charles K, Ahern R, King DM, Eisen T, Corringham R, DeWitte M, Balkwill F, Gore M
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2007 Oct 10;25(29):4542-9
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2007 Oct 10;25(29):4542-9
Ultrasensitive flow-based immunoassays using single-molecule counting.
Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
Clinical chemistry 2007 Nov;53(11):1990-5
Clinical chemistry 2007 Nov;53(11):1990-5
Lymphocytes and synovial fluid fibroblasts support osteoclastogenesis through RANKL, TNFalpha, and IL-7 in an in vitro model derived from human psoriatic arthritis.
Colucci S, Brunetti G, Cantatore FP, Oranger A, Mori G, Quarta L, Cirulli N, Mancini L, Corrado A, Grassi FR, Grano M
The Journal of pathology 2007 May;212(1):47-55
The Journal of pathology 2007 May;212(1):47-55
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood 2007 May 15;109(10):4272-9
Blood 2007 May 15;109(10):4272-9
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
AIDS (London, England) 2007 Jun 19;21(10):1263-72
AIDS (London, England) 2007 Jun 19;21(10):1263-72
Interleukin-10 modulates pro-apoptotic effects of TNF-alpha in human articular chondrocytes in vitro.
John T, Müller RD, Oberholzer A, Zreiqat H, Kohl B, Ertel W, Hostmann A, Tschoeke SK, Schulze-Tanzil G
Cytokine 2007 Dec;40(3):226-34
Cytokine 2007 Dec;40(3):226-34
Increased ENA-78 in the follicular fluid of patients with endometriosis.
Wunder DM, Mueller MD, Birkhäuser MH, Bersinger NA
Acta obstetricia et gynecologica Scandinavica 2006;85(3):336-42
Acta obstetricia et gynecologica Scandinavica 2006;85(3):336-42
Sequential induction of angiogenic growth factors by TNF-alpha in choroidal endothelial cells.
Hangai M, He S, Hoffmann S, Lim JI, Ryan SJ, Hinton DR
Journal of neuroimmunology 2006 Feb;171(1-2):45-56
Journal of neuroimmunology 2006 Feb;171(1-2):45-56
Selective regulation of IL-10 signaling and function by zymosan.
Du Z, Kelly E, Mecklenbräuker I, Agle L, Herrero C, Paik P, Ivashkiv LB
Journal of immunology (Baltimore, Md. : 1950) 2006 Apr 15;176(8):4785-92
Journal of immunology (Baltimore, Md. : 1950) 2006 Apr 15;176(8):4785-92
True infliximab resistance in rheumatoid arthritis: a role for lymphotoxin alpha?
Buch MH, Conaghan PG, Quinn MA, Bingham SJ, Veale D, Emery P
Annals of the rheumatic diseases 2004 Oct;63(10):1344-6
Annals of the rheumatic diseases 2004 Oct;63(10):1344-6
IFN-alpha priming results in a gain of proinflammatory function by IL-10: implications for systemic lupus erythematosus pathogenesis.
Sharif MN, Tassiulas I, Hu Y, Mecklenbräuker I, Tarakhovsky A, Ivashkiv LB
Journal of immunology (Baltimore, Md. : 1950) 2004 May 15;172(10):6476-81
Journal of immunology (Baltimore, Md. : 1950) 2004 May 15;172(10):6476-81
Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
Phillips TM
Electrophoresis 2004 Jun;25(10-11):1652-9
Electrophoresis 2004 Jun;25(10-11):1652-9
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Copeland S, Siddiqui J, Remick D
Journal of immunological methods 2004 Jan;284(1-2):99-106
Journal of immunological methods 2004 Jan;284(1-2):99-106
trans fatty acids and systemic inflammation in heart failure.
Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
The American journal of clinical nutrition 2004 Dec;80(6):1521-5
The American journal of clinical nutrition 2004 Dec;80(6):1521-5
Reprogramming of IL-10 activity and signaling by IFN-gamma.
Herrero C, Hu X, Li WP, Samuels S, Sharif MN, Kotenko S, Ivashkiv LB
Journal of immunology (Baltimore, Md. : 1950) 2003 Nov 15;171(10):5034-41
Journal of immunology (Baltimore, Md. : 1950) 2003 Nov 15;171(10):5034-41
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Recombinant Human TNF-alpha by Western Blot. Western blot shows 25 ng of Recombinant Human TNF-alpha (Catalog # 210-TA), Recombinant Mouse TNF-alpha aa 80-235 (Catalog # 410-MT), Recombinant Rat TNF-alpha (Catalog # 510-RT), and Recombinant Human APRIL/TNFSF13 (Catalog # 5860-AP). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human TNF-alpha Monoclonal Antibody (Catalog # MAB610) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TNF-alpha at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TNF-alpha in Human PBMCs. TNF-alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Mouse Anti-Human TNF-alpha Monoclonal Antibody (Catalog # MAB610) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Human TNF-alpha Antibody. Recombinant Human TNF-alpha (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-alpha (0.25 ng/mL) is neutral-ized (green line) by increasing concentrations of Mouse Anti-Human TNF-alpha Monoclonal Antibody (Catalog # MAB610). The ND50 is typically 0.01-0.04 µg/mL in the presence of the metabolic inhibitor actinomycin D.
