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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF1814 - Provider product page
- Provider
- R&D Systems
- Product name
- Equine TNF-alpha Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects equine TNF-alpha in direct ELISAs and Western blots. In Western blots, approximately 35% cross-reactivity with recombinant mouse TNF-alpha and recombinant rat TNF-alpha is observed.
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_001075288- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TNF-alpha in Equine PBMCs. TNF-alpha was detected in immersion fixed equine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Equine TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1814) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Equine TNF-alpha Antibody. Recombinant Equine TNF-alpha (Catalog # 1814-ET) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Equine TNF-alpha (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1814). The ND50 is typically 0.01-0.03 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
