502803

antibody from BioLegend
Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2
Provider product page for 502803
Western blot
ELISA
Blocking/Neutralizing
Other assay

Antibody data

Product number
502803 - Provider product page
Provider
BioLegend
Proper citation
BioLegend Cat#502803, RRID:AB_315251
Product name
LEAF™ Purified anti-human TNF-รก [MAb1]
Antibody type
Monoclonal
Antigen
E. coli-expressed, recombinant human TNF-α
Description
TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. The MAb1 antibody can neutralize the bioactivity of natural or recombinant TNF-α.
Reactivity
Human
Host
Mouse
Conjugate
Unconjugated
Isotype
IgG
Vial size
50 µg
Concentration
1 mg/ml
Storage
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
TNF protein structure - 502803 shown in red.