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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [17]
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- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- NB600-587 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-587, RRID:AB_10000823
- Product name
- Rabbit Polyclonal TNF-alpha Antibody
- Antibody type
- Polyclonal
- Description
- Delipidation and Defibrination. The antiserum is directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNG beta (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Simian
- Host
- Rabbit
- Vial size
- 0.2 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Astrocyte-Conditioned Medium Protects Prefrontal Cortical Neurons from Glutamate-Induced Cell Death by Inhibiting TNF-α Expression.
Dietary polyphenols as a safe and novel intervention for modulating pain associated with intervertebral disc degeneration in an in-vivo rat model.
Angiogenic and MMPs modulatory effects of icariin improved cutaneous wound healing in rats.
Traumatic brain injury-induced downregulation of Nrf2 activates inflammatory response and apoptotic cell death.
Chick Embryo: A Preclinical Model for Understanding Ischemia-Reperfusion Mechanism.
Protective effect of the standardized leaf extract of Ginkgo biloba (EGb761) against hypertension-induced renal injury in rats.
Inhibiting tumor necrosis factor-alpha at time of induced intervertebral disc injury limits long-term pain and degeneration in a rat model.
Effects of curcumin and melatonin on bone formation in orthopedically expanded suture in rats: A biochemical, histological and immunohistochemical study.
Obesity and p16INK4A Downregulation Activate Breast Adipocytes and Promote Their Protumorigenicity.
Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection.
Inhibitory Effect of Pycnogenol(®) on Airway Inflammation in Ovalbumin-Induced Allergic Rhinitis.
Adipocytes play an etiological role in the podocytopathy of high-fat diet-fed rats.
Loss of α(E)-catenin promotes Fas mediated apoptosis in tubular epithelial cells.
Inhibition of immunoproteasome reduces infarction volume and attenuates inflammatory reaction in a rat model of ischemic stroke.
Characterization of immune cells and cytokine localization in the rat utero-placental unit mid- to late gestation.
Altered composition of liver proteasome assemblies contributes to enhanced proteasome activity in the exceptionally long-lived naked mole-rat.
Distinct eosinophil cytokine expression patterns in skin diseases - the possible existence of functionally different eosinophil subpopulations.
Song C, Wu YS, Yang ZY, Kalueff AV, Tsao YY, Dong Y, Su KP
Neuroimmunomodulation 2019;26(1):33-42
Neuroimmunomodulation 2019;26(1):33-42
Dietary polyphenols as a safe and novel intervention for modulating pain associated with intervertebral disc degeneration in an in-vivo rat model.
Lai A, Ho L, Evashwick-Rogler TW, Watanabe H, Salandra J, Winkelstein BA, Laudier D, Hecht AC, Pasinetti GM, Iatridis JC
PloS one 2019;14(10):e0223435
PloS one 2019;14(10):e0223435
Angiogenic and MMPs modulatory effects of icariin improved cutaneous wound healing in rats.
Singh WR, Devi HS, Kumawat S, Sadam A, Appukuttan AV, Patel MR, Lingaraju MC, Singh TU, Kumar D
European journal of pharmacology 2019 Sep 5;858:172466
European journal of pharmacology 2019 Sep 5;858:172466
Traumatic brain injury-induced downregulation of Nrf2 activates inflammatory response and apoptotic cell death.
Bhowmick S, D'Mello V, Caruso D, Abdul-Muneer PM
Journal of molecular medicine (Berlin, Germany) 2019 Dec;97(12):1627-1641
Journal of molecular medicine (Berlin, Germany) 2019 Dec;97(12):1627-1641
Chick Embryo: A Preclinical Model for Understanding Ischemia-Reperfusion Mechanism.
Fauzia E, Barbhuyan TK, Shrivastava AK, Kumar M, Garg P, Khan MA, Robertson AAB, Raza SS
Frontiers in pharmacology 2018;9:1034
Frontiers in pharmacology 2018;9:1034
Protective effect of the standardized leaf extract of Ginkgo biloba (EGb761) against hypertension-induced renal injury in rats.
Abdel-Zaher AO, Farghaly HSM, El-Refaiy AEM, Abd-Eldayem AM
Clinical and experimental hypertension (New York, N.Y. : 1993) 2018;40(8):703-714
Clinical and experimental hypertension (New York, N.Y. : 1993) 2018;40(8):703-714
Inhibiting tumor necrosis factor-alpha at time of induced intervertebral disc injury limits long-term pain and degeneration in a rat model.
Evashwick-Rogler TW, Lai A, Watanabe H, Salandra JM, Winkelstein BA, Cho SK, Hecht AC, Iatridis JC
JOR spine 2018 Jun;1(2)
JOR spine 2018 Jun;1(2)
Effects of curcumin and melatonin on bone formation in orthopedically expanded suture in rats: A biochemical, histological and immunohistochemical study.
Cesur MG, Gülle K, Şirin FB, Akpolat M, Öğrenim G, Alkan A, Cesur G
Orthodontics & craniofacial research 2018 Jun 21;
Orthodontics & craniofacial research 2018 Jun 21;
Obesity and p16INK4A Downregulation Activate Breast Adipocytes and Promote Their Protumorigenicity.
Al-Khalaf HH, Amir M, Al-Mohanna F, Tulbah A, Al-Sayed A, Aboussekhra A
Molecular and cellular biology 2017 Sep 1;37(17)
Molecular and cellular biology 2017 Sep 1;37(17)
Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection.
Rossi AF, Cadamuro AC, Biselli-Périco JM, Leite KR, Severino FE, Reis PP, Cordeiro JA, Silva AE
Cellular microbiology 2016 Oct;18(10):1444-58
Cellular microbiology 2016 Oct;18(10):1444-58
Inhibitory Effect of Pycnogenol(®) on Airway Inflammation in Ovalbumin-Induced Allergic Rhinitis.
Günel C, Demirci B, Eryılmaz A, Yılmaz M, Meteoğlu İ, Ömürlü İK, Başal Y
Balkan medical journal 2016 Nov;33(6):620-626
Balkan medical journal 2016 Nov;33(6):620-626
Adipocytes play an etiological role in the podocytopathy of high-fat diet-fed rats.
Chen JY, Jian DY, Lien CC, Lin YT, Ting CH, Chen LK, Hsu TC, Huang HM, Wu YT, Kuan TT, Chao YW, Wu LY, Huang SW, Juan CC
The Journal of endocrinology 2016 Nov;231(2):109-120
The Journal of endocrinology 2016 Nov;231(2):109-120
Loss of α(E)-catenin promotes Fas mediated apoptosis in tubular epithelial cells.
Wang X, Parrish AR
Apoptosis : an international journal on programmed cell death 2015 Jul;20(7):921-9
Apoptosis : an international journal on programmed cell death 2015 Jul;20(7):921-9
Inhibition of immunoproteasome reduces infarction volume and attenuates inflammatory reaction in a rat model of ischemic stroke.
Chen X, Zhang X, Wang Y, Lei H, Su H, Zeng J, Pei Z, Huang R
Cell death & disease 2015 Jan 29;6:e1626
Cell death & disease 2015 Jan 29;6:e1626
Characterization of immune cells and cytokine localization in the rat utero-placental unit mid- to late gestation.
Tessier DR, Raha S, Holloway AC, Yockell-Lelièvre J, Tayade C, Gruslin A
Journal of reproductive immunology 2015 Aug;110:89-101
Journal of reproductive immunology 2015 Aug;110:89-101
Altered composition of liver proteasome assemblies contributes to enhanced proteasome activity in the exceptionally long-lived naked mole-rat.
Rodriguez KA, Edrey YH, Osmulski P, Gaczynska M, Buffenstein R
PloS one 2012;7(5):e35890
PloS one 2012;7(5):e35890
Distinct eosinophil cytokine expression patterns in skin diseases - the possible existence of functionally different eosinophil subpopulations.
Roth N, Städler S, Lemann M, Hösli S, Simon HU, Simon D
Allergy 2011 Nov;66(11):1477-86
Allergy 2011 Nov;66(11):1477-86
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TNF-alpha Antibody - Azide Free [NB600-587] - Expression of TNF-alpha, IL-1beta and NF-kappaB in the brains of MCAO rats. Western blot confirmed that protein levels of TNF-alpha increased as early as 4 h after reperfusion, further increased at 24 h, peaked at 72 h and decreased at 7 days, respectively (*P
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TNF-alpha Antibody - Azide Free [NB600-587] - Expression of TNF-alpha, IL-1beta and NF-kappaB in the brains of MCAO rats. Western blot confirmed that protein levels of TNF-alpha increased as early as 4 h after reperfusion, further increased at 24 h, peaked at 72 h and decreased at 7 days, respectively (*P
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TNF-alpha Antibody - Azide Free [NB600-587] - Analysis using Azide/BSA FREE version of NB600-587. Membrane blocked in 1% BSA-TBS-T 30 min RT, 1:1000 dilution in 1% BSA-TBS-T 4C.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TNF-alpha Antibody [NB600-587] - Analysis using Azide/BSA FREE version of NB600-587. Staining of human colon diluted 1:100 and incubated with the sample for 2 hours at 20C. Alexa Fluor(R) 680 goat polyclonal secondary antibody was used diluted 1:5000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: TNF-alpha Antibody - Azide Free [NB600-587] - Staining of formalin/PFA-fixed paraffin-embedded sections of human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum. Primary antibody was diluted 1:100 and incubated with samples for 24 hours at 4C. HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody.
