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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- TA307936 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal antibody to HICE1 (HAUS augmin-like complex, subunit 8)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody to HICE1 (HAUS augmin-like complex, subunit 8)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- HAUS8
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- 0.17mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB to detect cellular Hice1 and Hice1-GFP expressed in human osteosarcoma U2OS cells (left image), and Hice1 upon siRNA treatment (right image), using TA307936 at 1:1000 dilution. Nuclear matrix protein p84 is a loading control, blotted with p84 antibody
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sample. A: His-Hice1 (2x). B: His-Hice1 (1x). 7.5% SDS PAGE. TA307936 diluted at 1:500
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of human osteosarcoma cell line U2OS, using HICE1(TA307936) antibody at 1:50-1:200 dilution.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IP-WB assay to show that Hice1 co-immunoprecipitated with other Augmin components HAUS6, HAUS2 and HAUS1 in U2OS cells.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Human lymph tissue, using HICE1 (TA307936) antibody at 1:50 dilution.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Human lymph tissue, using HICE1 (TA307936) antibody at 1:50 dilution.
- Validation comment
- IHC
