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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- NBP2-42849 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP2-42849, RRID:AB_2665500
- Product name
- Rabbit Polyclonal HAUS8 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HAUS8 Antibody [NBP2-42849] - Detection of cellular Hice1 and Hice1-GFP expressed in human osteosarcoma U2OS cells (left image), and Hice1 upon siRNA treatment (right image). Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HAUS8 Antibody [NBP2-42849] - Analysis of A: His-Hice1 (2x) B: His-Hice1 (1x) 7.5% SDS PAGE diluted at 1:500.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: HAUS8 Antibody [NBP2-42849] - Analysis of Hice1 co-immunoprecipitated with other Augmin components HAUS6, HAUS2 and HAUS1 in U2OS cells.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HAUS8 Antibody [NBP2-42849] - Human lymph tissue, using HICE1 antibody at 1:50 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HAUS8 Antibody [NBP2-42849] - Human lymph tissue, using HICE1 antibody at 1:50 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
