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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- MAB2061 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB2061, RRID:AB_1715211
- Product name
- VAMP3 monoclonal antibody, clone k2A2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant VAMP3.
- Isotype
- IgG
- Antibody clone number
- k2A2
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Tetanus neurotoxin-mediated cleavage of cellubrevin impairs epithelial cell migration and integrin-dependent cell adhesion.
VAMP2, but not VAMP3/cellubrevin, mediates insulin-dependent incorporation of GLUT4 into the plasma membrane of L6 myoblasts.
Syntaxin 4, VAMP2, and/or VAMP3/cellubrevin are functional target membrane and vesicle SNAP receptors for insulin-stimulated GLUT4 translocation in adipocytes.
Proux-Gillardeaux V, Gavard J, Irinopoulou T, Mège RM, Galli T
Proceedings of the National Academy of Sciences of the United States of America 2005 May 3;102(18):6362-7
Proceedings of the National Academy of Sciences of the United States of America 2005 May 3;102(18):6362-7
VAMP2, but not VAMP3/cellubrevin, mediates insulin-dependent incorporation of GLUT4 into the plasma membrane of L6 myoblasts.
Randhawa VK, Bilan PJ, Khayat ZA, Daneman N, Liu Z, Ramlal T, Volchuk A, Peng XR, Coppola T, Regazzi R, Trimble WS, Klip A
Molecular biology of the cell 2000 Jul;11(7):2403-17
Molecular biology of the cell 2000 Jul;11(7):2403-17
Syntaxin 4, VAMP2, and/or VAMP3/cellubrevin are functional target membrane and vesicle SNAP receptors for insulin-stimulated GLUT4 translocation in adipocytes.
Olson AL, Knight JB, Pessin JE
Molecular and cellular biology 1997 May;17(5):2425-35
Molecular and cellular biology 1997 May;17(5):2425-35
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- The extract of mouse lung tissue (40 ug) was resolved by SDS-PAGE and probed with VAMP3 monoclonal antibody, clone K2A2 (Cat # MAB2061) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
