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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
- Other assay [1]
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Validation data
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- Product number
- UM800019 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM800019, RRID:AB_2629132
- Product name
- Purified HE4 (WFDC2) mouse monoclonal antibody,clone UMAB88
- Antibody type
- Monoclonal
- Description
- Purified HE4 (WFDC2) mouse monoclonal antibody,clone UMAB88
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- WFDC2
- Isotype
- IgG
- Antibody clone number
- UMAB88
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis of HT-29 cell lysate (35ug) by using anti-WFDC2 monoclonal antibody (Clone UMAB88)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:1000.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-WFDC2 mouse monoclonal antibody. (UM800019; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-WFDC2 mouse monoclonal antibody. (UM800019; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded 3 human ovarian cancer using anti-HE4 clone UMAB88 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800018 requires HIER with with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The tumor cells show membrane and cytoplasmic staining.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded 3 human endometrial cancer using anti-HE4 clone UMAB88 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800018 requires HIER with with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The tumor cells show membrane and cytoplasmic staining.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human lung cancer using anti-HE4 clone UMAB88 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800018 requires HIER with with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The tumor cells show membrane and cytoplasmic staining.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-WFDC2 mouse monoclonal antibody (UM800019). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
- Validation comment
- 10K-CHIP
