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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00009138-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00009138-M03, RRID:AB_714670
- Product name
- ARHGEF1 monoclonal antibody (M03), clone 4C4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant ARHGEF1.
- Antigen sequence
CRPGPEGQLAATALRKVLSLKQLLFPAEEDNGAGP
PRDGDGVPGGGPLSPARTQEIQENLLSLEETMKQL
EELEEEFCRLRPLLSQLGGNSVPQPGCT- Isotype
- IgG
- Antibody clone number
- 4C4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Loss of Lsc/p115 protein leads to neuronal hypoplasia in the esophagus and an achalasia-like phenotype in mice.
Zizer E, Beilke S, Bäuerle T, Schilling K, Möhnle U, Adler G, Fischer KD, Wagner M
Gastroenterology 2010 Oct;139(4):1344-54
Gastroenterology 2010 Oct;139(4):1344-54
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ARHGEF1 monoclonal antibody (M03), clone 4C4 Western Blot analysis of ARHGEF1 expression in K-562 ( Cat # L009V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ARHGEF1 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to ARHGEF1 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
