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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-34744 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CMTM6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, HeLa, HepG2, Jurkat, Raji, NCI-H929, mouse placenta, rat testis.
- Concentration
- 1.24 mg/mL
Submitted references Impact of CKLF-like MARVEL transmembrane domain containing 6 (CMTM6) expression in gastric cancer.
Nishi M, Shimada M, Yoshikawa K, Higashijima J, Tokunaga T, Kashihara H, Takasu C, Eto S, Yoshimoto T
The journal of medical investigation : JMI 2021;68(3.4):362-367
The journal of medical investigation : JMI 2021;68(3.4):362-367
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CMTM6 using 30 µg of HepG2 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a CMTM6 polyclonal antibody (Product # PA5-34744) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CMTM6 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CMTM6 Polyclonal Antibody (Product # PA5-34744) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CMTM6 was performed by separating 50 µg of mouse tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CMTM6 Polyclonal Antibody (Product # PA5-34744) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CMTM6 was performed by separating 50 µg of rat tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CMTM6 Polyclonal Antibody (Product # PA5-34744) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CMTM6 Polyclonal Antibody (Product # PA5-34744). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CMTM6 Polyclonal Antibody (Product # PA5-34744) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CMTM6 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CMTM6 Polyclonal Antibody (Product # PA5-34744) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CMTM6 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CMTM6 Polyclonal Antibody (Product # PA5-34744) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CMTM6 in methanol-fixed A431 cells using a CMTM6 polyclonal antibody (Product # PA5-34744) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded TW2.6 xenograft, using CMTM6 (Product # PA5-34744) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
