NBP1-31069

antibody from Novus Biologicals

Targeting: VPS35 FLJ10752, MEM3, PARK17

Immunocytochemistry

Antibody data

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Validation data

Product number: NBP1-31069 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NBP1-31069, RRID:AB_2272915
Product name: Rabbit Polyclonal VPS35 Antibody
Antibody type: Polyclonal
Description: Antigen Affinity-purified.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 0.1 ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

VPS35 protein structure - NBP1-31069 shown in red.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: VPS35 Antibody [NBP1-31069] - Sample(30 ug of whole cell lysate)A:H1299B:HeLa S3 C:Hep G2 D:MOLT4 E:Raji 7. 5% SDS PAGE, antibody diluted at 1:500.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: VPS35 Antibody [NBP1-31069] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: VPS35 Antibody [NBP1-31069] - A. 30 ug A549 whole cell extract B. 30 ug H1299 whole cell extract C. 30 ug HCT116 whole cell extract 7.5% SDS-PAGE VPS35 antibody [C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: VPS35 Antibody [NBP1-31069] - A. 50 ug rat brain lysate/extract 7.5% SDS-PAGE VPS35 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: VPS35 Antibody [NBP1-31069] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.