HPA017856

antibody from Atlas Antibodies

Targeting: AS3MT CYT19

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA017856

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA017856, RRID:AB_1847420

Product name

Anti-AS3MT

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

VLQEAYRVLKHGGELYFSDVYTSLELPEEIRTHKV
LWGECLGGALYWKELAVLAQKIGFCPPRLVTANLI
TIQNKELERVIGDCRFVSATFRLFKHSKTGPTKRC
QVIYNGGITGHEKELMFDANFTFKEGEIVEVDEET
AAILK


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human parathyroid gland and colon tissues using HPA017856 antibody. Corresponding AS3MT RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human small intestine shows cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human parathyroid gland shows cytoplasmic positivity in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human breast shows weak cytoplasmic positivity in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in Leydig cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows no cytoplasmic positivity in glandular cells as expected.

Sample type

HUMAN