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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- GTX102051 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102051, RRID:AB_11176166
- Product name
- PUF60 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE GTX102051 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PUF60 antibody detects PUF60 protein at nucleus by immunofluorescent analysis.Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PUF60 protein stained by PUF60 antibody (GTX102051) diluted at 1:500.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PUF60 antibody detects PUF60 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PUF60 protein stained by PUF60 antibody (GTX102051) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PUF60 antibody detects PUF60 protein at nucleus on U251 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U251 xenograft. PUF60 antibody (GTX102051) dilution: 1:500.
