PAB22109

antibody from Abnova Corporation

Targeting: PARP10 ARTD10, FLJ14464

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB22109

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB22109, RRID:AB_10964086

Product name

PARP10 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant PARP10.

Antigen sequence

PHYDILEPEELAENTSGGDHPSTQGPRATKHALLR
TGGLVTALQGAGTVTMGSGEEPGQSGTSLRTGPMV
QGRGIMTTGSGQEPGQSGTSL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with PARP10 polyclonal antibody (Cat # PAB22109) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with PARP10 polyclonal antibody (Cat # PAB22109) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and golgi apparatus.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human thyroid gland with PARP10 polyclonal antibody (Cat # PAB22109) shows distinct cytoplasmic positivity in glandular cells at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)