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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB-220-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human PDGF-BB Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human PDGF-BB in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant human (rh) PDGF-AB is observed, approximately 50% cross-reactivity with recombinant rat PDGF-B and natural porcine PDGF is observed, and less than 10% cross-reactivity with rhPDGF-A is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P01127- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Long noncoding RNA LERFS negatively regulates rheumatoid synovial aggression and proliferation.
Hypoxia Differentially Regulates Arterial and Venous Smooth Muscle Cell Migration.
Zou Y, Xu S, Xiao Y, Qiu Q, Shi M, Wang J, Liang L, Zhan Z, Yang X, Olsen N, Zheng SG, Xu H
The Journal of clinical investigation 2018 Oct 1;128(10):4510-4524
The Journal of clinical investigation 2018 Oct 1;128(10):4510-4524
Hypoxia Differentially Regulates Arterial and Venous Smooth Muscle Cell Migration.
Chanakira A, Kir D, Barke RA, Santilli SM, Ramakrishnan S, Roy S
PloS one 2015;10(9):e0138587
PloS one 2015;10(9):e0138587
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by PDGF-BB and Neutralization by Human PDGF-BB Antibody. Recombinant Human PDGF-BB (Catalog # 220-BB) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human PDGF-BB (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-BB Polyclonal Antibody (Catalog # AB-220-NA). The ND50 is typically 0.25-1.5 µg/mL.
