Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- AF815 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Bag-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Bag-1 in Western blots. Does not react with human Bag-1.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Bag-1 by Western Blot. Western blot shows lysates of CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.6 µg/mL of Goat Anti-Mouse Bag-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF815) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Bag-1 at approximately 32 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Immunoprecipitation of Mouse Bag-1. Bag-1 was immunoprecipitated from lysates (3 x 106 cells) of CTLL-2 mouse cytotoxic T cell line and A431 human epithelial carcinoma cell line following incubation with 5 µg Goat Anti-Mouse Bag-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF815) for 1 hour at 4 ºC. Bag-1-antibody complexes were absorbed using Protein G (Sigma). Immunoprecipitated Bag-1 was detected by Western blot using 5 µg/mL Mouse Bag-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF815). The leftmost lane represents a control experiment where the antibody was incubated with no cell lysate. View our recommended buffer recipes for immunoprecipitation.