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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [5]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00000573-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000573-M02, RRID:AB_537068
- Product name
- Mouse Monoclonal Bag-1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. BAG1 - BCL2-associated athanogene
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - analysis of BAG1 expression in transfected 293T cell line by BAG1 monoclonal antibody (M02), clone 2D3. Lane 1: BAG1 transfected lysate (38.878 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in Jurkat.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in PC-12.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in LNCaP.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3 Analysis of BAG1 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Bag-1 Antibody (2D3) [H00000573-M02] - Detection limit for recombinant GST tagged BAG1 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Bag-1 Antibody (2D3) [H00000573-M02] - Analysis of monoclonal antibody to BAG1 on HeLa cell. Antibody concentration 35 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Bag-1 Antibody (2D3) [H00000573-M02] - Analysis of monoclonal antibody to BAG1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
