orb21478

antibody from Biorbyt

Targeting: CD79A IGA, MB-1

No application information provided.

Antibody Data

Product number

orb21478

Provider

Biorbyt

Proper citation

Biorbyt Cat#orb21478, RRID:AB_10941395

Product name

Goat IgG Against Rat IgG1 IgG2A IgG2B IgG2C

Antibody type

Polyclonal

Description

Purified IgG fraction of polyclonal goat antiserum to rat IgG, Fc specific. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:50 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.

Host

Goat

Conjugate

Unconjugated

Vial size

10 mg

Concentration

IgG protein concentration 1 mg/ml. No foreign proteins added.

Storage

The lyophilised IgG fraction is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.