orb22214

antibody from Biorbyt

Targeting: CD79A IGA, MB-1

No application information provided.

Antibody Data

Product number

orb22214

Provider

Biorbyt

Proper citation

Biorbyt Cat#orb22214, RRID:AB_10920258

Product name

Mouse Ascites Against Human IgG4

Antibody type

Monoclonal

Description

Monoclonal mouse antibody to human IgG4, subclass specific. To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG4, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Host

Mouse

Conjugate

Unconjugated

Vial size

500 μg

Concentration

IgG concentration is 1. mg/ml. No foreign proteins added.

Storage

The lyophilised product is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. Reconstitute the lyophilised product by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at 4°C, not refrozen, and preferably used the same day.